Name: PhosphoWorks™ Fluorimetric ADP Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 21655
Price: 415 USD
Availability: InStock

PhosphoWorks™ Fluorimetric ADP Assay Kit

Red Fluorescence

PhosphoWorks™ Fluorimetric ADP Assay Kit *Red Fluorescence* is a fluorimetric assay kit for quantifying ADP formation or depletion in kinase reactions.

Detection Principle: Measures ADP formation proportional to kinase phosphotransferase activity.
Quantitation Range: Typically measures 0.05–30 µM ADP.
Workflow: Homogeneous, continuous assay adaptable to automation.
Incubation: Requires 15–60 minutes at room temperature.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm using solid black plates.

<p>The detection of protein kinase A with PhosphoWorks™ Fluorimetric ADP Assay Kit. The kinase was incubated in the presence of ATP and kemptide peptide substrate for 30 minutes, and ADP generation was detected after 30 minutes incubation using the Amplite® Fluorimetric Kinase Assay Kit.</p>

Protocol

SDS

Catalog	Size	Price	Quantity
21655	100 Tests	Price

Overview

ADP is involved in many biological reactions such as protein kinases. Our ADP assay kit can be used for assaying protein kinase reactions universally by monitoring ADP formation, which is directly proportional to enzyme phosphotransferase activity and is measured fluorimetrically. This kit provides a fast, simple and homogeneous assay for measuring ADP formation or depletion. The assay is continuous, and can be easily adapted to automation. The kit is convenient, requiring minimal hands-on time. Protein kinases are of interest to researchers involved in drug discovery due to their broad relevance to diseases such as cancer and other proliferative diseases, inflammatory diseases, metabolic disorders and neurological diseases. Most of commercial protein kinase assay kits are either based on monitoring of phosphopeptide formation or ATP deletion. Our ADP assay kit is more robust for assaying protein kinases than most of commercial kinase assay kits.

Citations

View all 4 citations: Citation Explorer

Discovery of Non-ATP-Competitive Inhibitors of Polo-like Kinase 1

Authors:

Yun, Taikangxiang and Qin, Tan and Liu, Ying and Lai, Luhua

Journal:

ChemMedChem (2016): 713--717

Cell polarity kinase MST4 cooperates with cAMP-dependent kinase to orchestrate histamine-stimulated acid secretion in gastric parietal cells

Authors:

Jiang, Hao and Wang, Wenwen and Zhang, Yin and Yao, William W and Jiang, Jiying and Qin, Bo and Yao, Wendy Y and Liu, Fusheng and Wu, Huihui and Ward, Tarsha L and others, undefined

Journal:

Journal of Biological Chemistry (2015): 28272--28285

Regulation of NDR1 activity by PLK1 ensures proper spindle orientation in mitosis

Authors:

Yan, Maomao and Chu, Lingluo and Qin, Bo and Wang, Zhikai and Liu, Xing and Jin, Changjiang and Zhang, Guanglan and Gomez, Marta and Hergovich, Alex and er , undefined and Chen, Zhengjun and others, undefined

Journal:

Scientific reports (2015): 10449

Trypanosoma brucei vacuolar transporter chaperone 4 (TbVtc4) is an acidocalcisome polyphosphate kinase required for in vivo infection

Authors:

L, undefined and er, Noelia and Ulrich, Paul N and Docampo, Roberto

Journal:

Journal of Biological Chemistry (2013): 34205--34216

References

View all 36 references: Citation Explorer

The ADP/ATP ratio: A novel predictive assay for quality assessment of isolated pancreatic islets

Authors:

Goto M, Holgersson J, Kumagai-Braesch M, Korsgren O.

Journal:

Am J Transplant (2006): 2483

Negative correlation between poly-ADP-ribosylation of spleen cell histone proteins and initial duration of dimethylnitrosamine exposure to mice in vivo measured by Western blot immunoprobe assay: a possible biomarker for cancer detection

Authors:

Devi BJ, Schneeweiss FH, Sharan RN.

Journal:

Cancer Detect Prev (2005): 66

A novel fluorometric assay for ADP-ribose pyrophosphatase activity

Authors:

Song EK, Park HJ, Kim JS, Lee HH, Kim UH, Han MK.

Journal:

J Biochem Biophys Methods (2005): 161

An assay for adenosine 5'-diphosphate (ADP)-glucose pyrophosphorylase that measures the synthesis of radioactive ADP-glucose with glycogen synthase

Authors:

Yep A, Bejar CM, Ballicora MA, Dubay JR, Iglesias AA, Preiss J.

Journal:

Anal Biochem (2004): 52

A nonradiometric, high-throughput assay for poly(ADP-ribose) glycohydrolase (PARG): application to inhibitor identification and evaluation

Authors:

Putt KS, Hergenrother PJ.

Journal:

Anal Biochem (2004): 256

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 12, 2026

Component A: ADP Sensor Buffer	1 vial (2 mL)
Component B1: ADP Sensor I (Light-sensitive)	1 vial powder
Component B2: ADP Sensor II	1 vial (1 mL)
Component B3: DMSO	1 vial (100 µL)
Component C: ADP Standard	1 vial
Component D: ADP Assay Buffer	1 vial (10 mL)