Name: PhosphoWorks™ Luminometric ATP Assay Kit *Extended Luminescence*
Brand: AAT Bioquest
SKU: 21608
Availability: InStock

PhosphoWorks™ Luminometric ATP Assay Kit

Extended Luminescence

PhosphoWorks™ Luminometric ATP Assay Kit *Extended Luminescence* is a luminometric assay kit for quantifying ATP and assessing cell proliferation and cytotoxicity in mammalian cells.

Detection Principle: Measures ATP through a firefly luciferase-based luminescent reaction.
Signal Advantage: Provides stable luminescence for up to 4 hours.
Sensitivity: Detects ATP down to 0.1 nM in AAT’s demonstrated dose-response assay.
Workflow: Homogeneous assay requiring no mixing or separation.
Plate Format: Supports 96- or 384-well luminescence assays.

<p>CHO-K1 cell number was measured with the PhosphoWorks™ Luminescence ATP Assay Kit on a 96-well white plate using a NOVOstar plate reader (BMG Labtech). The luminescence signal for CHO-K1 cells down to 100 cells per well was monitored for up to 2 hours (Z’ factor = 0.6). The integrated time was 1 second.</p>

Protocol

Catalog	Size	Price	Quantity
21608	10 Plates	Price
21609	1 Plate	Price

Overview

Adenosine triphosphate (ATP) plays a fundamental role in cellular energenics, metabolic regulation and cellular signaling. The PhosphoWorks™ ATP Assay Kit provides a fast, simple and homogeneous luminescence assay for the determination of cell proliferation and cytotoxicity in mammalian cells. The assay can be performed in a convenient 96-well and 384-well microtiter-plate format. The high sensitivity of this assay permits the detection of ATP in many biological systems, environmental samples and foods. This PhosphoWorks ATP Assay Kit has the stable luminescence signal as long as 4 hours. It has stable luminescence with no mixing or separations required, and formulated to have minimal hands-on time.

Citations

View all 14 citations: Citation Explorer

Palmitic Acid Exerts Anti-Tumorigenic Activities by Modulating Cellular Stress and Lipid Droplet Formation in Endometrial Cancer

Authors:

Zhao, Ziyi and Wang, Jiandong and Kong, Weimin and Newton, Meredith A and Burkett, Wesley C and Sun, Wenchuan and Buckingham, Lindsey and O’Donnell, Jillian and Suo, Hongyan and Deng, Boer and others,

Journal:

Biomolecules (2024): 601

Knocking out Fkbp51 decreases CCl4-induced liver injury through enhancement of mitochondrial function and Parkin activity

Authors:

Qiu, Bin and Zhong, Zhaohui and Dou, Longyu and Xu, Yuxue and Zou, Yi and Weldon, Korri and Wang, Jun and Zhang, Lingling and Liu, Ming and Williams, Kent E and others,

Journal:

Cell \& Bioscience (2024): 1

Coronary Endothelium No-Reflow Injury Is Associated with ROS-Modified Mitochondrial Fission through the JNK-Drp1 Signaling Pathway

Authors:

Chen, Yi and Liu, Chen and Zhou, Peng and Li, Jiannan and Zhao, Xiaoxiao and Wang, Ying and Chen, Runzhen and Song, Li and Zhao, Hanjun and Yan, Hongbing

Journal:

Oxidative Medicine and Cellular Longevity (2021)

KD025 Shifts Pulmonary Endothelial Cell Bioenergetics and Decreases Baseline Lung Permeability

Authors:

Lee, Ji Young and Stevens, Reece P and Kash, Mary and Zhou, Chun and Koloteva, Anna and Renema, Phoibe and Paudel, Sunita S and Stevens, Troy

Journal:

American Journal of Respiratory Cell and Molecular Biology (2020)

High throughput cell-based assay for identification of glycolate oxidase inhibitors as a potential treatment for Primary Hyperoxaluria Type 1

Authors:

Wang, Mengqiao and Xu, Miao and Long, Yan and Fargue, Sonia and Southall, Noel and Hu, Xin and McKew, John C and Danpure, Christopher J and Zheng, Wei

Journal:

Scientific Reports (2016)

References

View all 108 references: Citation Explorer

Cell-surface-localized ATP detection with immobilized firefly luciferase

Authors:

Nakamura M, Mie M, Funabashi H, Yamamoto K, Ando J, Kobatake E.

Journal:

Anal Biochem (2006): 61

Basic evaluation for new antimicrobial susceptibility testing of Mycobacterium leprae by bioluminescence assay (ATP method)

Authors:

Yamazaki T, Gidoh M, Matsuoka M.

Journal:

Nihon Hansenbyo Gakkai Zasshi (2006): 227

An efficient method for quantitative determination of cellular ATP synthetic activity

Authors:

Hara KY, Mori H.

Journal:

J Biomol Screen (2006): 310

Ca2+ oscillations stimulate an ATP increase during fertilization of mouse eggs

Authors:

Campbell K, Swann K.

Journal:

Dev Biol (2006): 225

Cells die with increased cytosolic ATP during apoptosis: a bioluminescence study with intracellular luciferase

Authors:

Zamaraeva MV, Sabirov RZ, Maeno E, Ando-Akatsuka Y, Bessonova SV, Okada Y.

Journal:

Cell Death Differ (2005): 1390

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Luminescence microplate reader
Recommended plate	Solid white

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 12, 2026

Component A: ATP Monitoring Enzyme	1 vial
Component B: ATP Sensor (Light-sensitive)	10 vials
Component C: Reaction Buffer	2 vials (50 mL/vial)

Component A: ATP Monitoring Enzyme	1 vial
Component B: ATP Sensor	1 vial
Component C: Reaction Buffer	1 vial (10 mL)
Component C: Reaction Buffer	1 vial (10 mL)