Name: PhosphoWorks™ Luminometric ATP Assay Kit *Maximized Luminescence*
Brand: AAT Bioquest
SKU: 21610
Availability: InStock

PhosphoWorks™ Luminometric ATP Assay Kit

Maximized Luminescence

PhosphoWorks™ Luminometric ATP Assay Kit *Maximized Luminescence* is a luminometric assay kit for quantifying ATP and assessing cell proliferation and cytotoxicity in mammalian cells.

Detection Principle: Measures ATP through a luminescent enzyme-based reaction.
Sensitivity: Detects as few as 10 cells/well.
Signal Advantage: Provides stable, maximized luminescence with minimal hands-on time.
Workflow: Homogeneous assay with no separation step.
Plate Format: Supports 96- or 384-well luminescence assays.

CHO-K1 cell number was measured with PhosphoWorks™ Luminescence ATP Assay Kit on a 96-well white plate using a NOVOstar plate reader (BMG Labtech). The integration time was 1 sec.

Protocol

Catalog	Size	Price	Quantity
21610	1 Plate	Price
21621	10 Plates	Price

Overview

Adenosine triphosphate (ATP) plays a fundamental role in cellular energenics, metabolic regulation and cellular signaling. The PhosphoWorks™ ATP Assay Kit provides a fast, simple and homogeneous luminescence assay for the determination of cell proliferation and cytotoxicity in mammalian cells. The assay can be performed in a convenient 96-well and 384-well microtiter-plate format. The high sensitivity of this assay permits the detection of ATP in many biological systems, environmental samples and foods. This PhosphoWorks ATP Assay Kit can detect as low as 10 cells/well. It has stable luminescence with no mixing or separations required, and formulated to have minimal hands-on time.

Citations

View all 13 citations: Citation Explorer

Palmitic Acid Exerts Anti-Tumorigenic Activities by Modulating Cellular Stress and Lipid Droplet Formation in Endometrial Cancer

Authors:

Zhao, Ziyi and Wang, Jiandong and Kong, Weimin and Newton, Meredith A and Burkett, Wesley C and Sun, Wenchuan and Buckingham, Lindsey and O’Donnell, Jillian and Suo, Hongyan and Deng, Boer and others,

Journal:

Biomolecules (2024): 601

Knocking out Fkbp51 decreases CCl4-induced liver injury through enhancement of mitochondrial function and Parkin activity

Authors:

Qiu, Bin and Zhong, Zhaohui and Dou, Longyu and Xu, Yuxue and Zou, Yi and Weldon, Korri and Wang, Jun and Zhang, Lingling and Liu, Ming and Williams, Kent E and others,

Journal:

Cell \& Bioscience (2024): 1

Hepatitis B Surface Antigen Activates Unfolded Protein Response in Forming Ground Glass Hepatocytes of Chronic Hepatitis B

Authors:

Li, Yao and Xia, Yuchen and Cheng, Xiaoming and Kleiner, David E and Hewitt, Stephen M and Sproch, Julia and Li, Tong and Zhuang, Hui and Liang, T Jake

Journal:

Viruses (2019): 386

The Different Effects of Atorvastatin and Pravastatin on Cell Death and PARP Activity in Pancreatic NIT-1 Cells

Authors:

Chen, Ya-Hui and Chen, Yi-Chun and Liu, Chin-San and Hsieh, Ming-Chia

Journal:

Journal of Diabetes Research (2016)

NT1014, a novel biguanide, inhibits ovarian cancer growth in vitro and in vivo

Authors:

Zhang, Lu and Han, Jianjun and Jackson, Am and a L , undefined and Clark, Leslie N and Kilgore, Joshua and Guo, Hui and Livingston, Nick and Batchelor, Kenneth and Yin, Yajie and Gilliam, Timothy P and others, undefined

Journal:

Journal of Hematology & Oncology (2016): 91

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Luminescence microplate reader
Recommended plate	Solid white

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Page updated on October 12, 2026

Component A: ATP Monitoring Enzyme	1 vial
Component B: ATP Sensor (Light-sensitive)	1 vial
Component C: Reaction Buffer	1 vial (10 mL)

Component A: ATP Monitoring Enzyme	1 vial
Component B: ATP Sensor (Light-sensitive)	10 vials
Component C: Reaction Buffer	2 vials (50 mL/vial)