PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit *Enhanced Selectivity*

PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit

Enhanced Selectivity

PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit *Enhanced Selectivity* is a fluorimetric assay kit for quantifying pyrophosphate (PPi) using a microplate assay.

Detection Principle: Uses a proprietary fluorogenic sensor with signal proportional to PPi concentration.
Sensitivity: Detects PPi down to 1 µM (100 pmol/well) after 10 minutes.
Selectivity: Shows high selectivity for PPi over phosphate and ATP.
Workflow: Simple 10–30 minute room-temperature incubation before fluorescence measurement.
Instrument Compatibility: Fluorescence detection at Ex/Em 370/470 nm using solid black plates.

Pyrophosphate, ATP and phosphate dose responses were measured with PhosphoWorks™ Fluoremetric Pyrophosphate Assay Kit in a solid black 96-well plate using a fluorescence microplate reader.

Protocol

SDS

Catalog	Size	Price	Quantity
21614	200 Tests	Price

Overview

Pyrophosphate (PPi) is produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters. Our PhosphoWorks™ Pyrophosphate Assay Kit provides the most robust spectrophotometric method for measuring pyrophosphate. This kit uses our proprietary fluorogenic pyrophosphate sensor that has its fluorescence intensity proportionally dependent upon the concentration of pyrophosphate. The PPi sensor used in the kit has quite high selectivity to PPi compared to phosphate and ATP. Our assay is much easier and more robust than the enzyme-coupling pyrophosphate methods that require at least two enzymes for their pyrophosphate detections. The kit provides all the essential components for assaying pyrophosphate. This kit has been successfully used in high throughput screening (HTS). Please inquire special HTS bulk package discount for the screening of >10,000 assays.

Citations

View all 14 citations: Citation Explorer

Covalent allosteric inhibition of AARS1 lactyltransferase

Authors:

Huang, Yunyuan and Liu, Siying and Tian, Luyang and Tang, Yang and Dou, Yilin and Wang, Huiling and Zheng, Tao and Wang, Mingyang and Li, Runhao and Wang, Zhi and others,

Journal:

Nature Communications (2026)

Targeting tRNA-dependent tyrosine usage unveils a metabolic vulnerability in hepatocellular carcinoma

Authors:

Zhang, Hongli and Wang, Zixuan and Zhao, Yiqi and Deng, Xiya and Zhang, Jian and Shang, Fei and Zhang, Hongjie and Liu, Yan and Wang, Hongbo and Yang, Yating and others,

Journal:

Nature Communications (2026): 2244

Modulation of host ATP levels by secreted bacterial effectors

Authors:

He, Chunlin and Li, Chuang and Liu, Yao and Chen, Tao-Tao and Li, Chunxiuli and Chu, Xiao and Liu, Shuxin and Wang, Lidong and Zhang, Yong and Ouyang, Songying and others,

Journal:

Nature Communications (2025): 1--15

Inorganic Pyrophosphate at Serum Concentration May Not Be Able to Inhibit Mineralization: A Study in Aqueous Solutions and Serum

Authors:

Cheng, Yuxuan and Ru, Jing and Feng, Chaobo and Liu, Xiaohao and Zeng, Hua and Tan, Shuo and Chen, Xi and Chen, Feng and Lu, Bing-Qiang

Journal:

ACS Omega (2024)

Metabolic enzyme UAP1 mediates IRF3 pyrophosphorylation to facilitate innate immune response

Authors:

Yang, Shuai and Jin, Shouheng and Xian, Huifang and Zhao, Zhiyao and Wang, Liqiu and Wu, Yaoxing and Zhou, Liang and Li, Mengqiu and Cui, Jun

Journal:

Molecular Cell (2023)

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	370 nm
Emission	470 nm
Cutoff	455 nm
Recommended plate	Solid black

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Email	sales@aatbio.com
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Page updated on October 28, 2026

Component A: Assay Buffer	1 bottle (25 mL)
Component B: PPi Sensor	1 vial (lyophilized powder)
Component C: Pyrophosphate Standard	1 vial (1 mL, 50 mM)
Component D: DMSO	1 vial (100 µL)