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PhosphoWorks™ Fluorimetric ATP Assay Kit
Adenosine triphosphate (ATP) plays a fundamental role in cellular energetics, metabolic regulation and cellular signaling. It is referred as the "molecular unit of currency" of intracellular energy transfer to drive many processes and chemical synthesis in living cells. ATP also serves as a signaling molecule for cell communication and plays an important role in DNA and RNA synthesis. AAT Bioquest offers a variety of bioluminescence assay kits to determine nanomolar (nM) range of ATP with recombinant firefly luciferase (Cat# 21610 & 21609). These kits require luminescence plate readers, are frequently used for cell viability or cytotoxicity assays. PhosphoWorks™ Fluorimetric ATP Assay Kit is based on a serial ATP-induced enzyme coupled reactions to produce hydrogen peroxide, which is spectrophotometrically quantified with our Amplite® Red Substrate. The assay can detect ~0.4 µM of ATP in a 100 µL reaction volume with minimal interference from ADP and AMP. It provides a robust, simple and convenient assay for measuring ATP levels in biological samples. The PhosphoWorks™ Fluorimetric ATP Assay is complementary to our luciferase-based ATP assay kits.
ATP dose response was measured with PhosphoWorks™ Fluorimetric ATP Assay Kit in a 96-well solid black plate using a Germini microplate reader (Molecular Devices).
ATP dose response was measured with PhosphoWorks™ Fluorimetric ATP Assay Kit in a 96-well solid black plate using a Germini microplate reader (Molecular Devices).
CatalogSize
Price
Quantity
21620100 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Fluorescence microplate reader
Excitation540 mn
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on October 13, 2025