Name: Portelite™ Fluorimetric DNA Quantitation Kit with Broad Dynamic Range *Optimized for Cytocite™ and Qubit™ Fluorometers*
Brand: AAT Bioquest
SKU: 17665
Price: 92 USD
Availability: InStock

Portelite™ Fluorimetric DNA Quantitation Kit with Broad Dynamic Range

Optimized for Cytocite™ and Qubit™ Fluorometers

Portelite™ Fluorimetric DNA Quantitation Kit with Broad Dynamic Range is a fluorimetric assay kit for quantifying double-stranded DNA (dsDNA) using CytoCite™ or Qubit™ fluorometers.

Detection Principle: Helixyte™ Green BR increases fluorescence upon binding dsDNA.
Quantitation Range: Optimized for 10 pg/µL to 10 ng/µL dsDNA.
Selectivity: Highly selective for dsDNA over RNA.
Dynamic Range: Linear over three orders of magnitude.
Workflow: 2-minute room-temperature incubation with detection at Ex/Em 480/530 nm.

DNA standard curve generated using Portelite™ Fluorimetric DNA Quantitation Kit with Broad Dynamic Range. Fluorescence intensity was quantified using green fluorescence channel, regression model was calculated using linear fit.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17665	100 Tests	Price

Overview

DNA Quantitation is a very important task in DNA sample preparations for various analyses. Portelite™ Fluorimetric DNA Quantitation Kit provides a rapid method to quantify dsDNA with Helixyte™ Green BR using a hand-held fluorometer. It is optimized for Cytocite™ and Qubit™ fluorometers. Portelite™ Fluorimetric DNA Quantitation assay is linear over three orders of magnitude. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is optimized for measuring dsDNA concentrations from 10 pg/µL to 10 ng/µL. Helixyte™ Green BR exhibits large fluorescence enhancement upon binding to dsDNA, and it is a few magnitudes more sensitive than UV absorbance readings.

Citations

View all 32 citations: Citation Explorer

A new reporter design based on DNA origami nanostructures for quantification of short oligonucleotides using microbeads

Authors:

Choi, Y., Schmidt, C., Tinnefeld, P., Bald, I., Rodiger, S.

Journal:

Sci Rep (2019): 4769

Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification

Authors:

Stehr, F., Stein, J., Schueder, F., Schwille, P., Jungmann, R.

Journal:

Nat Commun (2019): 1268

Effects of Quantification Methods, Isolation Kits, Plasma Biobanking, and Hemolysis on Cell-Free DNA Analysis in Plasma

Authors:

Streleckiene, G., Forster, M., Inciuraite, R., Lukosevicius, R., Skieceviciene, J.

Journal:

Biopreserv Biobank (2019): ersion="1.0" encoding="UTF-8" ?>17645.enlEndN

A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity

Authors:

Sheppard, E. C., Rogers, S., Harmer, N. J., Chahwan, R.

Journal:

Sci Rep (2019): 8853

Molecular-Recognition-Based DNA Nanodevices for Enhancing the Direct Visualization and Quantification of Single Vesicles of Tumor Exosomes in Plasma Microsamples

Authors:

He, D., Ho, S. L., Chan, H. N., Wang, H., Hai, L., He, X., Wang, K., Li, H. W.

Journal:

Anal Chem (2019): 2768-2775

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Qubit Fluorometer
Excitation	480 nm
Emission	530 nm
Instrument specification(s)	0.2 mL PCR vial

CytoCite Fluorometer
Excitation	480 nm
Emission	530 nm
Instrument specification(s)	0.2 mL PCR vial

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2026

Component A: Helixyte™ Green BR	1 vial (0.25 mL, 200X in DMSO)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: DNA Standard BR #1	1 vial (1 mL, Calf thymus DNA: 0 ng/uL)
Component D: DNA Standard BR #2	1 vial (1 mL, Calf thymus DNA: 100 ng/uL)