Name: Purified Anti-human CD3 Antibody *HIT3a*
Brand: AAT Bioquest
SKU: 10030000
Price: 172 USD
Availability: InStock

Purified Anti-human CD3 Antibody

HIT3a

HIT3a is an anti-human monoclonal antibody that recognizes the CD3e antigen. CD3e (sometimes referred to as T cell antigen receptor complex or T3E) is a 20 kD member of the Ig superfamily that is found on the surface of cells like T cells. CD3 acts in essential cellular pathways, namely, the G protein-coupled receptor signaling pathway, apoptotic signaling pathway and T cell receptor signaling pathway. In addition, in certain organisms, it positively regulates T cell anergy, acts to positively regulate cell-matrix adhesion and is involved in the positive regulation of calcium-mediated signaling. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography.

General packaging for catalog 10030000. Actual shipped packaging may vary.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
10030000	100 ug	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd3e antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Physical properties

Molecular weight	N/A
Solvent	water

Antibody properties

Other names	T3E
Clone	HIT3a
Host	mouse
Isotype	mouse IgG2a
Reactivity	human
Application	Flow Cytometry (FACS); ELISA; IHC; Western Blot

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 26, 2025