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Purified Anti-human CD33 Antibody *HI33a*

The HI33a monoclonal antibody recognizes human CD33, a 67 kD single-pass type i membrane protein frequently expressed on the surface of monocytes, neutrophils, dendritic cells and mast cells. In some organisms, CD33 plays a role in the downregulation of calcium ion transport, is a suppressor of tumor necrosis factor production and is an inhibitor of interleukin-1 beta production. Additionally, it has been closely linked to vital biological processes such as signal transduction, especially immune response-inhibiting signal transduction. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands such as α-2 and 6- linked Sialic acid. CD33 is a fairly uncommon antibody target, with a little more than 8000 publications in the last decade. Even still, CD33 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of neuroscience. This antibody was purified through affinity chromatography.

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd33 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on October 14, 2024

Ordering information

Price
Unit size
Catalog Number10330000
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Antibody properties

Other namesSiglec-3; gp67
CloneHI33a
Hostmouse
Isotypemouse IgG2a
Reactivityhuman
ApplicationFlow Cytometry (FACS); ELISA; IHC; Western Blot

Physical properties

Molecular weight

N/A

Solvent

water

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
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