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Purified Anti-human CD4 Antibody *HIT4a*

HIT4a is an anti-human monoclonal antibody that targets the CD4 antigen. CD4 (alternatively called Leu3a or T4) is a 55 kD single-pass type I membrane protein that is expressed on the surface of cells such as granulocytes, T cells and macrophages. CD4 is involved with key cellular pathways, namely, the enzyme linked receptor protein signaling pathway, transmembrane receptor protein tyrosine kinase signaling pathway and cytokine-mediated signaling pathway. Moreover, in many organisms, it is involved in the positive regulation of monocyte differentiation, upregulates MAPK cascade and is an enhancer of ERK1 and ERK2 cascade. CD4 has been associated with vital biological processes like immune response, particularly adaptive immune response, and is associated with a variety of biologically interesting macromolecules/ligands, for example, Lck, IL-16, MHC Class II and gp120. CD4 is a very popular antibody target, with over 180000 publications in the last decade. CD4 is essential for immunology research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography.

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd4 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on October 14, 2024

Ordering information

Price
Unit size
Catalog Number10040000
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Antibody properties

Other namesLeu-3; T4
CloneHIT4a
Hostmouse
Isotypemouse IgG2b
Reactivityhuman
ApplicationFlow Cytometry (FACS); ELISA; IHC; Western Blot

Physical properties

Molecular weight

N/A

Solvent

water

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
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