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ReadiLink™ xtra Rapid Cy7 Antibody Labeling Kit
BSA-Compatible
ReadiLink™ xtra rapid antibody labeling kits require essentially only 2 simple mixing steps without a column purification needed. Specially formulated Cy7 used in this ReadiLink™ kit is quite stable and shows good reactivity and selectivity with antibodies. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of specially formulated Cy7 dye provided in the kit is optimized for labeling ~50 µg antibody. ReadiLink™ xtra Cy7 rapid antibody labeling kit provides a convenient and robust method to label monoclonal and polyclonal antibodies with NIR fluorescent Cy7 fluorophore. Cy7 is one of the most used fluorophores for labeling antibodies.

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ xtra Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

Immunofluorescence staining of tubulin in HeLa cells. HeLa cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 and blocked. Cells were then incubated with mouse anti-tubulin antibody and stained with a goat anti-mouse IgG labeled using the ReadiLink™ xtra Rapid Cy7 Antibody Labeling Kit (Cat No. 1973).
Immunofluorescence staining of tubulin in HeLa cells. HeLa cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 and blocked. Cells were then incubated with mouse anti-tubulin antibody and stained with a goat anti-mouse IgG labeled using the ReadiLink™ xtra Rapid Cy7 Antibody Labeling Kit (Cat No. 1973).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
19732 Labelings
Price
 
Spectral properties
Correction factor (260 nm)0.05
Correction factor (280 nm)0.036
Correction factor (482 nm)0.0005
Correction factor (565 nm)0.0193
Correction factor (650 nm)0.165
Extinction coefficient (cm -1 M -1)
250000
Excitation (nm)756
Emission (nm)779
Quantum yield
0.3
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 6, 2025