ReadiUse™ 6-Color Human TBNK Antibody Kit *Dry Reagent Format*

ReadiUse™ 6-Color Human TBNK Antibody Kit

Dry Reagent Format

ReadiUse™ 6-Color Human TBNK Antibody Kit is a dry-reagent flow cytometry kit for identifying and quantifying major human lymphocyte subsets in whole blood and PBMC samples.

Multiparameter Detection: Identifies CD4+ and CD8+ T cells, B cells, and NK cells.
Dry Reagent Format: Preloaded antibody cocktail simplifies sample preparation.
Lyse/No-Wash Workflow: Eliminates washing steps to reduce cell loss and variability.
Absolute Quantitation: Fluorescent counting beads enable absolute lymphocyte subset counts.
Viability Assessment: Includes a viability stain to distinguish live and dead cells.

Human peripheral blood was stained with the ReadiUse™ Human TBNK 6 Color Antibody Kit *Dry Reagent*. Cells were stained at room temperature for 20 minutes, lysed with 1X Lysis/Fix buffer for 15 minutes, and then analyzed by flow cytometry. Live cells were gated on NucPO-1 viability stain. Live cells are gated on CD45+ (2D1)-PerCP-iFluor® 680 for lymphocytes and then gated on CD3+ or CD3- (SK7) iFluor® 488 cell populations. CD3+ cells are shown using CD4 (SK3) PE-iFluor® 750 and CD8 (SK1) APC-iFluor® 750 markers. CD3- cells are shown using CD19 (SJ25C1)-APC and CD56 (5.1H11) / CD16 (B73.1)-PE markers.

Protocol

COA

Catalog	Size	Price	Quantity
90010	5 Tests	Price
90011	25 Tests	Price

Overview

The ReadiUse™ 6-Color Human TBNK Antibody Kit for flow cytometry utilizes dried multicolor beads to delineate principal lymphocyte subsets - CD4+ T cells, CD8+ T cells, B cells, and NK cells – in whole blood specimens. Each TBNK dried reagent contains a cocktail of fluorescently-labeled monoclonal antibodies directed against human CD3, CD4, CD8, CD16, C19, CD45, and CD56. The kit is designed to be used in a "lyse/no-wash" format for quick and easy sample preparation, with less inconsistencies. Also included in this kit is a viability stain to identify dead cells and a precise number of fluorescent counting beads to determine absolute lymphocyte subset cell concentrations.

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 15, 2026

Component A: TBNK Dried Reagent	5 Tubes
Component B: 10X Lyse/Fix Buffer	1 Bottle (0.3 mL)

Component A: TBNK Dried Reagent	25 Tubes
Component B: 10X Lyse/Fix Buffer	5 Bottles (0.3 mL/bottle)