Name: Resorufin alpha-D-galactopyranoside
Brand: AAT Bioquest
SKU: 14021
Price: 348 USD
Availability: InStock

Resorufin alpha-D-galactopyranoside

Resorufin alpha-D-galactopyranoside is a sensitive fluorogenic substrate that generates a red fluorescent product (resorufin) upon interaction with alpha-galactosidase. It is used for measuring alpha-galactosidase activities and high throughput screening of alpha-galactosidase inhibitors. Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme is a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry's disease, a rare lysosomal storage disorder and sphingolipidosis that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
14021	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Resorufin alpha-D-galactopyranoside to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	266.432 µL	1.332 mL	2.664 mL	13.322 mL	26.643 mL
5 mM	53.286 µL	266.432 µL	532.864 µL	2.664 mL	5.329 mL
10 mM	26.643 µL	133.216 µL	266.432 µL	1.332 mL	2.664 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	375.33 g/mol	=	mL	x	mM	=	mmol

References

View all 12 references: Citation Explorer

Preparation of monodisperse and size-controlled poly(ethylene glycol) hydrogel nanoparticles using liposome templates

Authors:

An SY, Bui MP, Nam YJ, Han KN, Li CA, Choo J, Lee EK, Katoh S, Kumada Y, Seong GH.

Journal:

J Colloid Interface Sci (2009): 98

Synthesis and characterization of a new fluorogenic substrate for alpha-galactosidase

Authors:

Shi ZD, Motabar O, Goldin E, Liu K, Southall N, Sidransky E, Austin CP, Griffiths GL, Zheng W.

Journal:

Anal Bioanal Chem (2009): 1903

Determination of kinetic parameters, Km and kcat, with a single experiment on a chip

Authors:

Jambovane S, Duin EC, Kim SK, Hong JW.

Journal:

Anal Chem (2009): 3239

Fast mixing and reaction initiation control of single-enzyme kinetics in confined volumes

Authors:

Jung SY, Liu Y, Collier CP.

Journal:

Langmuir (2008): 4439

Photolithographic fabrication of poly(ethylene glycol) microstructures for hydrogel-based microreactors and spatially addressed microarrays

Authors:

Baek TJ, Kim NH, Choo J, Lee EK, Seong GH.

Journal:

J Microbiol Biotechnol (2007): 1826

Physical properties

Molecular weight	375.33
Solvent	DMSO

Spectral properties

Absorbance (nm)	570
Extinction coefficient (cm ^-1 M ^-1)	65000 ¹
Excitation (nm)	571
Emission (nm)	584
Quantum yield	0.75 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 12, 2025