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Resorufin alpha-D-galactopyranoside
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 375.33 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 570 |
| Extinction coefficient (cm -1 M -1) | 650001 |
| Excitation (nm) | 571 |
| Emission (nm) | 584 |
| Quantum yield | 0.751 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
Molecular weight 375.33 | Absorbance (nm) 570 | Extinction coefficient (cm -1 M -1) 650001 | Excitation (nm) 571 | Emission (nm) 584 | Quantum yield 0.751 |
Resorufin alpha-D-galactopyranoside is a sensitive fluorogenic substrate that generates a red fluorescent product (resorufin) upon interaction with alpha-galactosidase. It is used for measuring alpha-galactosidase activities and high throughput screening of alpha-galactosidase inhibitors. Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme is a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry's disease, a rare lysosomal storage disorder and sphingolipidosis that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Resorufin alpha-D-galactopyranoside to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 266.432 µL | 1.332 mL | 2.664 mL | 13.322 mL | 26.643 mL |
| 5 mM | 53.286 µL | 266.432 µL | 532.864 µL | 2.664 mL | 5.329 mL |
| 10 mM | 26.643 µL | 133.216 µL | 266.432 µL | 1.332 mL | 2.664 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 570 |
| Extinction coefficient (cm -1 M -1) | 650001 |
| Excitation (nm) | 571 |
| Emission (nm) | 584 |
| Quantum yield | 0.751 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Resorufin beta-D-galactopyranoside *CAS 95079-19-9* | 571 | 584 | 650001 | 0.751 |
| Resorufin alpha-D-glucopyranoside *CAS 136565-96-3* | 571 | 584 | 650001 | 0.751 |
Images
Figure 1. Chemical structure for Resorufin alpha-D-galactopyranoside
References
View all 12 references: Citation Explorer
Determination of kinetic parameters, Km and kcat, with a single experiment on a chip
Authors: Jambovane S, Duin EC, Kim SK, Hong JW.
Journal: Anal Chem (2009): 3239
Authors: Jambovane S, Duin EC, Kim SK, Hong JW.
Journal: Anal Chem (2009): 3239
Synthesis and characterization of a new fluorogenic substrate for alpha-galactosidase
Authors: Shi ZD, Motabar O, Goldin E, Liu K, Southall N, Sidransky E, Austin CP, Griffiths GL, Zheng W.
Journal: Anal Bioanal Chem (2009): 1903
Authors: Shi ZD, Motabar O, Goldin E, Liu K, Southall N, Sidransky E, Austin CP, Griffiths GL, Zheng W.
Journal: Anal Bioanal Chem (2009): 1903
Preparation of monodisperse and size-controlled poly(ethylene glycol) hydrogel nanoparticles using liposome templates
Authors: An SY, Bui MP, Nam YJ, Han KN, Li CA, Choo J, Lee EK, Katoh S, Kumada Y, Seong GH.
Journal: J Colloid Interface Sci (2009): 98
Authors: An SY, Bui MP, Nam YJ, Han KN, Li CA, Choo J, Lee EK, Katoh S, Kumada Y, Seong GH.
Journal: J Colloid Interface Sci (2009): 98
Fast mixing and reaction initiation control of single-enzyme kinetics in confined volumes
Authors: Jung SY, Liu Y, Collier CP.
Journal: Langmuir (2008): 4439
Authors: Jung SY, Liu Y, Collier CP.
Journal: Langmuir (2008): 4439
Photolithographic fabrication of poly(ethylene glycol) microstructures for hydrogel-based microreactors and spatially addressed microarrays
Authors: Baek TJ, Kim NH, Choo J, Lee EK, Seong GH.
Journal: J Microbiol Biotechnol (2007): 1826
Authors: Baek TJ, Kim NH, Choo J, Lee EK, Seong GH.
Journal: J Microbiol Biotechnol (2007): 1826
Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited
Authors: English BP, Min W, van Oijen AM, Lee KT, Luo G, Sun H, Cherayil BJ, Kou SC, Xie XS.
Journal: Nat Chem Biol (2006): 87
Authors: English BP, Min W, van Oijen AM, Lee KT, Luo G, Sun H, Cherayil BJ, Kou SC, Xie XS.
Journal: Nat Chem Biol (2006): 87
Galactose oxidase action on galactose containing glycolipids--a fluorescence method
Authors: Fortelius M, Mattjus P.
Journal: Chem Phys Lipids (2006): 103
Authors: Fortelius M, Mattjus P.
Journal: Chem Phys Lipids (2006): 103
Measurement of enzyme kinetics using a continuous-flow microfluidic system
Authors: Seong GH, Heo J, Crooks RM.
Journal: Anal Chem (2003): 3161
Authors: Seong GH, Heo J, Crooks RM.
Journal: Anal Chem (2003): 3161
Crystallization of beta-galactosidase does not reduce the range of activity of individual molecules
Authors: Shoemaker GK, Juers DH, Coombs JM, Matthews BW, Craig DB.
Journal: Biochemistry (2003): 1707
Authors: Shoemaker GK, Juers DH, Coombs JM, Matthews BW, Craig DB.
Journal: Biochemistry (2003): 1707
beta-galactosidase assay using capillary electrophoresis laser-induced fluorescence detection and resorufin-beta-D-galactopyranoside as substrate
Authors: Eggertson MJ, Craig DB.
Journal: Biomed Chromatogr (1999): 516
Authors: Eggertson MJ, Craig DB.
Journal: Biomed Chromatogr (1999): 516