Resorufin alpha-D-glucopyranoside *CAS 136565-96-3*

Resorufin alpha-D-glucopyranoside

CAS 136565-96-3

Resorufin alpha-D-glucopyranoside is a sensitive fluorogenic substrate that generates a red fluorescent product (resorufin) upon interaction with alpha-glucosidase. It is used for measuring alpha-glucosidase activities and high throughput screening of alpha-glucosidase inhibitors. Alpha-glucosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-glucolactosyl moieties from glycolipids and glycoproteins. Mutations in alpha-glucosidase cause accumulation of glycogen in lysosomes, resulting in Pompe disease, a lysosomal storage disorder. Small molecule chaperones that bind to enzyme proteins and correct the misfolding and mistrafficking of mutant proteins have emerged as a new therapeutic approach for the lysosomal storage disorders. In addition, alpha-glucosidase is a therapeutic target for type II diabetes, and alpha-glucosidase inhibitors have been used in the clinic as alternative treatments for this disease. Resorufin alpha-D-glucopyranoside is a new fluorogenic substrate for the alpha-glucosidase enzyme assay, resorufin alpha-d-glucopyranoside. The enzyme reaction product of this new substrate emits at a peak of 590 nm, reducing the interference from fluorescent compounds seen with the existing fluorogenic substrate, 4-methylumbelliferyl-alpha-D-glucopyranoside. Also, the enzyme kinetic assay can be carried out continuously without the addition of stop solution due to the lower pK(a) of the product of this substrate. Therefore, this new fluorogenic substrate is a useful tool for the alpha-glucosidase enzyme assay and will facilitate compound screening for the development of new therapies for Pompe disease.

Protocol

COA

Catalog	Size	Price	Quantity
14022	5 mg	Price
14023	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Resorufin alpha-D-glucopyranoside *CAS 136565-96-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	266.432 µL	1.332 mL	2.664 mL	13.322 mL	26.643 mL
5 mM	53.286 µL	266.432 µL	532.864 µL	2.664 mL	5.329 mL
10 mM	26.643 µL	133.216 µL	266.432 µL	1.332 mL	2.664 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	375.33 g/mol	=	mL	x	mM	=	mmol

Physical properties

Molecular weight	375.33
Solvent	DMSO

Spectral properties

Absorbance (nm)	570
Extinction coefficient (cm ^-1 M ^-1)	65000 ¹
Excitation (nm)	571
Emission (nm)	584
Quantum yield	0.75 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 7, 2025