Resorufin alpha-D-glucopyranoside *CAS 136565-96-3*

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Chemical structure for Resorufin alpha-D-glucopyranoside *CAS 136565-96-3*
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Ex/Em (nm)571/585
CAS #136565-96-3
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Category Enzyme Detection
Hydrolytic Enzymes
Related Reporter Gene Enzymes
Secondary Reagents
Resorufin alpha-D-glucopyranoside is a sensitive fluorogenic substrate that generates a red fluorescent product (resorufin) upon interaction with alpha-glucosidase. It is used for measuring alpha-glucosidase activities and high throughput screening of alpha-glucosidase inhibitors. Alpha-glucosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-glucolactosyl moieties from glycolipids and glycoproteins. Mutations in alpha-glucosidase cause accumulation of glycogen in lysosomes, resulting in Pompe disease, a lysosomal storage disorder. Small molecule chaperones that bind to enzyme proteins and correct the misfolding and mistrafficking of mutant proteins have emerged as a new therapeutic approach for the lysosomal storage disorders. In addition, alpha-glucosidase is a therapeutic target for type II diabetes, and alpha-glucosidase inhibitors have been used in the clinic as alternative treatments for this disease. Resorufin alpha-D-glucopyranoside is a new fluorogenic substrate for the alpha-glucosidase enzyme assay, resorufin alpha-d-glucopyranoside. The enzyme reaction product of this new substrate emits at a peak of 590 nm, reducing the interference from fluorescent compounds seen with the existing fluorogenic substrate, 4-methylumbelliferyl-alpha-D-glucopyranoside. Also, the enzyme kinetic assay can be carried out continuously without the addition of stop solution due to the lower pK(a) of the product of this substrate. Therefore, this new fluorogenic substrate is a useful tool for the alpha-glucosidase enzyme assay and will facilitate compound screening for the development of new therapies for Pompe disease.

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Table 1. Volume of DMSO needed to reconstitute specific mass of Resorufin alpha-D-glucopyranoside *CAS 136565-96-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

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References & Citations

A new resorufin-based alpha-glucosidase assay for high-throughput screening
Authors: Motabar O, Shi ZD, Goldin E, Liu K, Southall N, Sidransky E, Austin CP, Griffiths GL, Zheng W.
Journal: Anal Biochem (2009): 79

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