logo
AAT Bioquest

Resorufin benzyl ether *CAS 87687-02-3*

This fluorogenic resorufin ether is widely used for monitoring P450 activities in cell extracts and solutions. Cytochrome P450 oxidase (CYP450) is a large number of evolutionary related oxidative enzymes important in animal, plant, and bacterial physiology. Most cytochromes P450 (CYPs) have about 500 amino acids and a heme group at the active site.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Resorufin benzyl ether *CAS 87687-02-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM329.696 µL1.648 mL3.297 mL16.485 mL32.97 mL
5 mM65.939 µL329.696 µL659.391 µL3.297 mL6.594 mL
10 mM32.97 µL164.848 µL329.696 µL1.648 mL3.297 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yield
Resorufin ethyl ether *CAS 5725-91-7*5715846500010.751
Resorufin methyl ether *CAS 5725-89-3*5715846500010.751
Resorufin pentyl ether *CAS 87687-03-4*5715846500010.751

Citations

View all 1 citations: Citation Explorer
Pancreatic progenitor-derived hepatocytes are viable and functional in a 3D high density bioreactor culture system
Authors: Richter, M and Fairhall, EA and Hoffmann, SA and Tröbs, S and Knöspel, F and Probert, PME and Oakley, F and Stroux, A and Wright, MC and Zeilinger, K
Journal: Toxicology Research (2016): 278--290

References

View all 57 references: Citation Explorer
Methoxy-resorufin ether as an electrochemically active biological probe for cytochrome P450 O-demethylation
Authors: Jenkins AT, Dash HA, Boundy S, Halliwell CM, ffrench-Constant RH.
Journal: Bioelectrochemistry (2006): 67
Comparison of cytochrome P450 inhibition assays for drug discovery using human liver microsomes with LC-MS, rhCYP450 isozymes with fluorescence, and double cocktail with LC-MS
Authors: Di L, Kerns EH, Li SQ, Carter GT.
Journal: Int J Pharm. (2006)
Ginkgo biloba does not alter clearance of flurbiprofen, a cytochrome P450-2C9 substrate
Authors: Greenblatt DJ, von Moltke LL, Luo Y, Perloff ES, Horan KA, Bruce A, Reynolds RC, Harmatz JS, Avula B, Khan IA, Goldman P.
Journal: J Clin Pharmacol (2006): 214
Structure of microsomal cytochrome P450 2B4 complexed with the antifungal drug bifonazole: insight into P450 conformational plasticity and membrane interaction
Authors: Zhao Y, White MA, Muralidhara BK, Sun L, Halpert JR, Stout CD.
Journal: J Biol Chem (2006): 5973
Cytochrome P450 (CYP) inhibition screening: comparison of three tests
Authors: Turpeinen M, Korhonen LE, Tolonen A, Uusitalo J, Juvonen R, Raunio H, Pelkonen O.
Journal: Eur J Pharm Sci (2006): 130
Page updated on September 18, 2024

Ordering information

Price
Unit size
Catalog Number15020
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

303.31

Solvent

DMSO

Spectral properties

Absorbance (nm)

570

Extinction coefficient (cm -1 M -1)

650001

Excitation (nm)

571

Emission (nm)

584

Quantum yield

0.751

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

87687-02-3
Product Image
Product Image
Gallery Image 1