Name: Resorufin beta-D-galactopyranoside *CAS 95079-19-9*
Brand: AAT Bioquest
SKU: 14019
Price: 115 USD
Availability: InStock

Resorufin beta-D-galactopyranoside

CAS 95079-19-9

Resorufin beta-D-galactopyranoside is a fluorogenic galactosidase substrate that generates a red fluorescent product upon interaction with the enzyme. This enzyme substrate is widely used in high throughput screening of galactosidase inhibitors.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
14019	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Resorufin beta-D-galactopyranoside *CAS 95079-19-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	266.432 µL	1.332 mL	2.664 mL	13.322 mL	26.643 mL
5 mM	53.286 µL	266.432 µL	532.864 µL	2.664 mL	5.329 mL
10 mM	26.643 µL	133.216 µL	266.432 µL	1.332 mL	2.664 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	375.33 g/mol	=	mL	x	mM	=	mmol

References

View all 8 references: Citation Explorer

Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited

Authors:

English BP, Min W, van Oijen AM, Lee KT, Luo G, Sun H, Cherayil BJ, Kou SC, Xie XS.

Journal:

Nat Chem Biol (2006): 87

Crystallization of beta-galactosidase does not reduce the range of activity of individual molecules

Authors:

Shoemaker GK, Juers DH, Coombs JM, Matthews BW, Craig DB.

Journal:

Biochemistry (2003): 1707

Measurement of enzyme kinetics using a continuous-flow microfluidic system

Authors:

Seong GH, Heo J, Crooks RM.

Journal:

Anal Chem (2003): 3161

beta-galactosidase assay using capillary electrophoresis laser-induced fluorescence detection and resorufin-beta-D-galactopyranoside as substrate

Authors:

Eggertson MJ, Craig DB.

Journal:

Biomed Chromatogr (1999): 516

Microchip device for performing enzyme assays

Authors:

Hadd AG, Raymond DE, Halliwell JW, Jacobson SC, Ramsey JM.

Journal:

Anal Chem (1997): 3407

Physical properties

Molecular weight	375.33
Solvent	DMSO

Spectral properties

Absorbance (nm)	570
Extinction coefficient (cm ^-1 M ^-1)	65000 ¹
Excitation (nm)	571
Emission (nm)	584
Quantum yield	0.75 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	95079-19-9

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