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Resorufin methyl ether *CAS 5725-89-3*
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Physical properties
| Molecular weight | 227.21 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 570 |
| Extinction coefficient (cm -1 M -1) | 650001 |
| Excitation (nm) | 571 |
| Emission (nm) | 584 |
| Quantum yield | 0.751 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
CAS 5725-89-3 | Molecular weight 227.21 | Absorbance (nm) 570 | Extinction coefficient (cm -1 M -1) 650001 | Excitation (nm) 571 | Emission (nm) 584 | Quantum yield 0.751 |
This fluorogenic resorufin ether is widely used for monitoring P450 activities in cell extracts and solutions. Cytochrome P450 oxidase (CYP450) is a large number of evolutionary related oxidative enzymes important in animal, plant, and bacterial physiology. Most cytochromes P450 (CYPs) have about 500 amino acids and a heme group at the active site.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Resorufin methyl ether *CAS 5725-89-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 440.121 µL | 2.201 mL | 4.401 mL | 22.006 mL | 44.012 mL |
| 5 mM | 88.024 µL | 440.121 µL | 880.243 µL | 4.401 mL | 8.802 mL |
| 10 mM | 44.012 µL | 220.061 µL | 440.121 µL | 2.201 mL | 4.401 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 570 |
| Extinction coefficient (cm -1 M -1) | 650001 |
| Excitation (nm) | 571 |
| Emission (nm) | 584 |
| Quantum yield | 0.751 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Resorufin benzyl ether *CAS 87687-02-3* | 571 | 584 | 650001 | 0.751 |
| Resorufin ethyl ether *CAS 5725-91-7* | 571 | 584 | 650001 | 0.751 |
| Resorufin pentyl ether *CAS 87687-03-4* | 571 | 584 | 650001 | 0.751 |
Images
Figure 1. Chemical structure for Resorufin methyl ether *CAS 5725-89-3*
Citations
View all 1 citations: Citation Explorer
Pancreatic progenitor-derived hepatocytes are viable and functional in a 3D high density bioreactor culture system
Authors: Richter, M and Fairhall, EA and Hoffmann, SA and Tröbs, S and Knöspel, F and Probert, PME and Oakley, F and Stroux, A and Wright, MC and Zeilinger, K
Journal: Toxicology Research (2016): 278--290
Authors: Richter, M and Fairhall, EA and Hoffmann, SA and Tröbs, S and Knöspel, F and Probert, PME and Oakley, F and Stroux, A and Wright, MC and Zeilinger, K
Journal: Toxicology Research (2016): 278--290
References
View all 57 references: Citation Explorer
Induction of cytochrome P450 3A4 and P-glycoprotein by the isoxazolyl-penicillin antibiotic flucloxacillin
Authors: Huwyler J, Wright MB, Gutmann H, Drewe J.
Journal: Curr Drug Metab (2006): 119
Authors: Huwyler J, Wright MB, Gutmann H, Drewe J.
Journal: Curr Drug Metab (2006): 119
Luminogenic cytochrome P450 assays
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Journal: Expert Opin Drug Metab Toxicol (2006): 629
Authors: Cali JJ, Ma D, Sobol M, Simpson DJ, Frackman S, Good TD, Daily WJ, Liu D.
Journal: Expert Opin Drug Metab Toxicol (2006): 629
Kinetics and thermodynamics of ligand binding by cytochrome P450 3A4
Authors: Isin EM, Guengerich FP.
Journal: J Biol Chem (2006): 9127
Authors: Isin EM, Guengerich FP.
Journal: J Biol Chem (2006): 9127
Resolution of multiple substrate binding sites in cytochrome P450 3A4: the stoichiometry of the enzyme-substrate complexes probed by FRET and Job's titration
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Authors: Fern, undefined and o H, Halpert JR, Davydov DR.
Journal: Biochemistry (2006): 4199
Binding of 7-methoxy-4-(aminomethyl)-coumarin to wild-type and W128F mutant cytochrome P450 2D6 studied by time-resolved fluorescence spectroscopy
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Journal: Biochem J (2006): 635
Time-resolved fluorescence studies of heterotropic ligand binding to cytochrome P450 3A4
Authors: Lampe JN, Atkins WM.
Journal: Biochemistry (2006): 12204
Authors: Lampe JN, Atkins WM.
Journal: Biochemistry (2006): 12204
Methoxy-resorufin ether as an electrochemically active biological probe for cytochrome P450 O-demethylation
Authors: Jenkins AT, Dash HA, Boundy S, Halliwell CM, ffrench-Constant RH.
Journal: Bioelectrochemistry (2006): 67
Authors: Jenkins AT, Dash HA, Boundy S, Halliwell CM, ffrench-Constant RH.
Journal: Bioelectrochemistry (2006): 67
Cytochrome P450 (CYP) inhibition screening: comparison of three tests
Authors: Turpeinen M, Korhonen LE, Tolonen A, Uusitalo J, Juvonen R, Raunio H, Pelkonen O.
Journal: Eur J Pharm Sci (2006): 130
Authors: Turpeinen M, Korhonen LE, Tolonen A, Uusitalo J, Juvonen R, Raunio H, Pelkonen O.
Journal: Eur J Pharm Sci (2006): 130
Structure of microsomal cytochrome P450 2B4 complexed with the antifungal drug bifonazole: insight into P450 conformational plasticity and membrane interaction
Authors: Zhao Y, White MA, Muralidhara BK, Sun L, Halpert JR, Stout CD.
Journal: J Biol Chem (2006): 5973
Authors: Zhao Y, White MA, Muralidhara BK, Sun L, Halpert JR, Stout CD.
Journal: J Biol Chem (2006): 5973
Ginkgo biloba does not alter clearance of flurbiprofen, a cytochrome P450-2C9 substrate
Authors: Greenblatt DJ, von Moltke LL, Luo Y, Perloff ES, Horan KA, Bruce A, Reynolds RC, Harmatz JS, Avula B, Khan IA, Goldman P.
Journal: J Clin Pharmacol (2006): 214
Authors: Greenblatt DJ, von Moltke LL, Luo Y, Perloff ES, Horan KA, Bruce A, Reynolds RC, Harmatz JS, Avula B, Khan IA, Goldman P.
Journal: J Clin Pharmacol (2006): 214