Rhod-5N, tripotassium salt

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Telephone	1-800-990-8053
Fax	1-800-609-2943
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Physical properties

Dissociation constant (K_d, nM)	300000
Molecular weight	900.02
Solvent	Water

Spectral properties

Excitation (nm)	557
Emission (nm)	580

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

900.02

Dissociation constant (K_d, nM)

300000

Excitation (nm)

557

Emission (nm)

580

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-5N has a lower binding affinity for Ca2+ (Kd = ~320 µM) than any other BAPTA-based indicator and is suitable for Ca2+ measurements from 10 µM to 1 mM. Like the parent Rhod-2 indicator, Rhod-5N is essentially nonfluorescent in the absence of divalent cations and exhibits strong fluorescence enhancement with no spectral shift upon binding Ca2+.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Rhod-5N, tripotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	111.109 µL	555.543 µL	1.111 mL	5.555 mL	11.111 mL
5 mM	22.222 µL	111.109 µL	222.217 µL	1.111 mL	2.222 mL
10 mM	11.111 µL	55.554 µL	111.109 µL	555.543 µL	1.111 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	557
Emission (nm)	580

Product Family

Name	Excitation (nm)	Emission (nm)	Quantum yield
Rhod-2, tripotassium salt	553	577	0.1¹
Rhod-FF, tripotassium salt	553	577	-

Images

Figure 1. Chemical structure for Rhod-5N, tripotassium salt

Citations

View all 3 citations: Citation Explorer

Imaging Ca2+ Concentration and pH in Nanopores/Channels of Protein Crystals
Authors: Mori, Kazuo and Kuhn, Bernd
Journal: The Journal of Physical Chemistry B (2018): 9646--9653

Activation of mitochondrial transient receptor potential vanilloid 1 channel contributes to microglial migration
Authors: Miyake, Takahito and Shirakawa, Hisashi and Nakagawa, Takayuki and Kaneko, Shuji
Journal: Glia (2015): 1870--1882

ER stress in human hepatic cells treated with Efavirenz: mitochondria again
Authors: Apostolova, Nadezda and Gomez-Sucerquia, Leysa J and Alegre, Fern and o , undefined and Funes, Haryes A and Victor, Victor M and Barrachina, Maria D and Blas-Garcia, Ana and Esplugues, Juan V
Journal: Journal of hepatology (2013): 780--789

References

View all 2 references: Citation Explorer

Ionic calcium determination in skim milk with molecular probes and front-face fluorescence spectroscopy: simple linear regression
Authors: Gangidi RR, Metzger LE.
Journal: J Dairy Sci (2006): 4105

Measurement of limestone biodeterioration using the Ca2+ binding fluorochrome Rhod-5N
Authors: McNamara CJ, Perry TDt, Bearce K, Hern and ez-Duque G, Mitchell R.
Journal: J Microbiol Methods (2005): 245