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Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit
The Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit offers a fast, sensitive, and high-throughput method for screening and characterizing potential inhibitors of NE. This assay measures the NE activity based on hydrolysis of the substrate, producing a fluorescent product with Ex/Em=360/470nm. The amount of fluorescent product is directly proportional to the enzymatic activity of the neutrophil elastase present in the sample. Neutrophil elastase, also known as leukocyte elastase, ELANE, ELA2, elastase 2, neutrophil, elaszym, serine elastase, subtype human leukocyte elastase (HLE) is a cytotoxic Serine Protease with a broad substrate specificity. Azurophil granules of neutrophil store NE and release them in response to multiple stimuli like pathogens, immune complexes, or chemotactic agents such as PMA causing degradation of a range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. NE hydrolysis accounts for approximately 80% of the total protease hydrolysis activity in the human body. Disease implications of NE include cystic fibrosis, COPD, lung emphysema, rheumatoid arthritis, and adult respiratory distress syndrome. NE has also been associated with lung injury seen in COVID-19.
The NE Inhibitor dose response was measured using the Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit on a 96-well solid black microplate with a Gemini microplate reader (Molecular Devices) Ex/Em = 360/470 nm (Cutoff = 430 nm).
The NE Inhibitor dose response was measured using the Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit on a 96-well solid black microplate with a Gemini microplate reader (Molecular Devices) Ex/Em = 360/470 nm (Cutoff = 430 nm).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11802100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation360 nm
Emission470 nm
Cutoff430 nm
Recommended plateSolid black
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on November 5, 2025