Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit measures neutrophil elastase (NE) activity through hydrolysis of a fluorogenic substrate. Substrate cleavage generates a fluorescent product, allowing enzymatic activity to be monitored using a fluorescence microplate reader. Compounds that inhibit NE reduce fluorescent product formation relative to the uninhibited enzyme control.

The kit includes NE enzyme, a fluorogenic substrate, a reference inhibitor, and assay buffers. It supports 96-well and 384-well microplate formats, with recommended reader settings of Ex/Em = 360/470 nm. Enzyme concentration and compound exposure conditions require empirical optimization when evaluating different inhibitors. The kit supports biochemical screening and concentration-response characterization of potential neutrophil elastase inhibitors.