Name: Signal Guard™ 10X Rapid HRP reaction stopping solution
Brand: AAT Bioquest
SKU: 11019
Price: 118 USD
Availability: InStock

Signal Guard™ 10X Rapid HRP reaction stopping solution

Signal Guard™ 10X Rapid HRP Reaction Stopping Solution instantly stops peroxidase reactions while preserving signal stability, ensuring consistent results in HRP-based assays.

Assay Signal Stabilized Upon Adding: Assay Signal is stabilized upon adding the stop solution
Rapid Deactivation of HRP/Peroxidase Activity: Rapid deactivation of HRP/Peroxidase activity
Versatile Applications: Suitable for a wide range of research and diagnostic applications
Compatible With Both Chromogenic and Fluorogenic: Compatible with both chromogenic and fluorogenic HRP substrates

Formalin-fixed, paraffin-embedded (FFPE) human lung tissue sections were immunolabeled with CD4-XFD488 mAb, followed by incubation with HRP-conjugated goat anti-XFD488 IgG. To inhibit HRP enzymatic activity, sections were treated with Signal Guard™ 10X Rapid HRP Reaction Stopping Solution. Fluorescence was then developed using iFluor® 647 styramide (Cat. 45045) and visualized using a Cy5 filter set. Tissue sections pre-treated with Signal Guard™ prior to styramide application exhibited no detectable signal (Right), in contrast to untreated sections (Left), demonstrating effective inhibition of HRP activity by the stopping solution.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11019	0.5 mL	Price

Overview

Signal Guard™ 10X rapid HRP reaction stopping solution is designed to rapidly terminate peroxidase-mediated reactions. HRP-based assays are widely used in research and diagnostics. It is critical to ensure that HRP-coupled enzyme tests can be stopped at the desired time to have a highly reproducible and robust test, which requires a robust and fast HRP stopping reagent. AAT Bioquest developed the Signal Guard™ rapid HRP reagent to instantly stop peroxidase-based reactions. Most commercially available HRP stopping solutions are based on the competitive inhibition of the HRP, thus they are slow and ineffective. The Signal Guard™ rapid HRP stopping reagent disrupts the HRP redox cycle, which makes it versatile across a spectrum of HRP substrates. It is compatible with both chromogenic and fluorogenic HRP substrates.

References

View all 50 references: Citation Explorer

Z-scheme heterojunction with spatially separated dual redox-active sites for coupled carbon dioxide photoreduction and tetracycline photooxidation.

Authors:

Li, Shuang and Lin, Haili and Jia, Xuemei and Wang, Qianlong and Zhang, Haoyu and Zhang, Yujie and Feng, Xiaoxin and Chen, Shifu and Cao, Jing

Journal:

Journal of colloid and interface science (2025): 138218

Exo I-based cyclic digestion coupled with synergistic enhancement strategy for integrating dual-mode optical aptasensor platform.

Authors:

Li, Ming and Teng, Weipeng and Lu, Wenying and Sun, Mingna and Duan, Jinsheng and Qiu, Xuchun

Journal:

Talanta (2024): 126286

The Peroxidase-Coupled Assay to Measure MAO Enzymatic Activity.

Authors:

Reis, Joana and Binda, Claudia

Journal:

Methods in molecular biology (Clifton, N.J.) (2023): 23-34

Ultrasensitive electrochemical sensing platform for miRNA-21 detection based on manganese dioxide-gold nanoparticle nanoconjugates coupled with hybridization chain reaction and horseradish peroxidase signal amplification.

Authors:

Li, Mengyao and Zhang, Tingting and Zhang, Yuzhong

Journal:

The Analyst (2023): 2180-2188

Horseradish Peroxidase-Coupled Ag3 PO4 /BiVO4 Photoanode for Biophotoelectrocatalytic Degradation of Organic Contaminants.

Authors:

Liu, Jianqiao and Qin, Jin and Li, Shiquan and Yan, Kai and Zhang, Jingdong

Journal:

ChemSusChem (2023): e202202212

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C)

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Page updated on April 15, 2026