Signal Guard™ 10X Rapid HRP reaction stopping solution

Product key features

Rapid deactivation of HRP/Peroxidase activity
Assya Signal is stabilized upon adding the stop solution
Compatible with both chromogenic and fluorogenic HRP substrates

Product description

Signal Guard™ 10X rapid HRP reaction stopping solution is designed to rapidly terminate peroxidase-mediated reactions. HRP-based assays are widely used in research and diagnostics. It is critical to ensure that HRP-coupled enzyme tests can be stopped at the desired time to have a highly reproducible and robust test, which requires a robust and fast HRP stopping reagent. AAT Bioquest developed the Signal Guard™ rapid HRP reagent to instantly stop peroxidase-based reactions. Most commercially available HRP stopping solutions are based on the competitive inhibition of the HRP, thus they are slow and ineffective. The Signal Guard™ rapid HRP stopping reagent disrupts the HRP redox cycle, which makes it versatile across a spectrum of HRP substrates. It is compatible with both chromogenic and fluorogenic HRP substrates.

Example protocol

PREPARATION OF WORKING SOLUTION

Preparation of Working solution (1X)

Prepare a working solution by adding 100 μL of Signal Guard™ 10X Rapid HRP reaction stopping solution to 900 μL of PBS or a buffer of your choice.

Note: For optimal performance, use the working solution within a few hours of preparation.

Note: 10 mL of working solution is enough for 100 tests.

SAMPLE EXPERIMENTAL PROTOCOL

Sample Experimental Protocol

For biochemical assays requiring an in-solution stop, add an equal volume of Signal Guard™ 1X Rapid HRP Reaction Stopping Working Solution to each well at the desired time point. Incubate at room temperature for 5 minutes, then proceed with the next step of the assay.

For example, if each well contains 100 µL of assay mixture, add 100 µL of the 1X working solution to achieve a 1:1 ratio.
For tissue samples, add 100 µL volume of Signal Guard™ 10X Rapid HRP reaction stopping working solution (1X) directly onto the sample to ensure complete coverage. Incubate at 60 °C for 60 minutes, then wash thoroughly with PBS before proceeding to the next steps.

References

View all 50 references: Citation Explorer

Z-scheme heterojunction with spatially separated dual redox-active sites for coupled carbon dioxide photoreduction and tetracycline photooxidation.
Authors: Li, Shuang and Lin, Haili and Jia, Xuemei and Wang, Qianlong and Zhang, Haoyu and Zhang, Yujie and Feng, Xiaoxin and Chen, Shifu and Cao, Jing
Journal: Journal of colloid and interface science (2025): 138218

Exo I-based cyclic digestion coupled with synergistic enhancement strategy for integrating dual-mode optical aptasensor platform.
Authors: Li, Ming and Teng, Weipeng and Lu, Wenying and Sun, Mingna and Duan, Jinsheng and Qiu, Xuchun
Journal: Talanta (2024): 126286

The Peroxidase-Coupled Assay to Measure MAO Enzymatic Activity.
Authors: Reis, Joana and Binda, Claudia
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 23-34

Ultrasensitive electrochemical sensing platform for miRNA-21 detection based on manganese dioxide-gold nanoparticle nanoconjugates coupled with hybridization chain reaction and horseradish peroxidase signal amplification.
Authors: Li, Mengyao and Zhang, Tingting and Zhang, Yuzhong
Journal: The Analyst (2023): 2180-2188

Horseradish Peroxidase-Coupled Ag3 PO4 /BiVO4 Photoanode for Biophotoelectrocatalytic Degradation of Organic Contaminants.
Authors: Liu, Jianqiao and Qin, Jin and Li, Shiquan and Yan, Kai and Zhang, Jingdong
Journal: ChemSusChem (2023): e202202212

Page updated on July 16, 2025

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