SoNa™ 520
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Catalog Number | |
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Molecular weight | 720.72 |
Solvent | DMSO |
Excitation (nm) | 491 |
Emission (nm) | 511 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
SoNa™ 520 AM |
Overview | SDSProtocol |
Molecular weight 720.72 | Excitation (nm) 491 | Emission (nm) 511 |
Platform
Fluorescence microplate reader
Excitation | 490 nm |
Emission | 525 nm |
Cutoff | 515 nm |
Recommended plate | Solid black |
Example protocol
AT A GLANCE
Add 50 µL NaCl Standards or test samples
Add 50 µL SoNa™ 520 working solution.
Incubate at RT for 5-10 minutes
Monitor the fluorescence at Ex/Em=490/525 nm
The following protocol is an example for quantifying sodium content using SoNa™ 520. The DMSO stock solution should be handled with particular caution as DMSO is known to facilitate the entry of organic molecules into tissues.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add DMSO into SoNa™ 520 vial (Component A) to make 2 to 5 mM stock solution.
Note: Make a single unused SoNa™ 520 stock solution aliquot and store at ≤ -20 º C. Protect from light and avoid repeated freeze-thaw cycles.
PREPARATION OF STANDARD SOLUTIONS
https://www.aatbio.com/tools/serial-dilution/21320
PREPARATION OF WORKING SOLUTION
Prepare 10 to 20 µM SoNa™ 520 working solution into 5 mL of Tris Buffer (pH∼7.5). Protect the working solution from light by covering it with foil or placing it in the dark.
Note: For best results, this solution should be used within a few hours of its preparation.
Note: 5 mL of working solution is enough for 100 tests.
SAMPLE EXPERIMENTAL PROTOCOL
The following protocol only provides a guideline and should be modified according to your specific needs.
SS=NaCl Standards (SS1 - SS7, 40 to 0.625 mM, 2X dilutions); BL=Blank Control; TS=Test Samples
BL | BL | TS | TS |
SS1 | SS1 | ... | ... |
SS2 | SS2 | ... | ... |
SS3 | SS3 | ... | ... |
SS4 | SS4 | ... | ... |
SS5 | SS5 | ... | ... |
SS6 | SS6 | ... | ... |
SS7 | SS7 | ... | ... |
Well | Volume | Reagent |
SS1-SS7 | 50 µL | Serial dilutions (40 to 0.625 mM) |
BL | 50 µL | Tris Buffer |
TS | 50 µL | Sample |
Prepare NaCl standards (SS), blank controls (BL), and test samples (TS) according to the layout provided in Tables 1 and 2. For a 384-well plate, use 25 µL of reagent per well instead of 50 µL.
Add 50 µL of SoNa™ 520 working solution to each well of NaCl standards, blank control, and test samples to make the assay volume of 100 µL/well. For a 384-well plate, add 25 µL into each well instead, for a total volume of 50 µL/well.
Incubate the reaction at room temperature for 5 to 10 minutes, protected from light.
Monitor the fluorescence increase with a fluorescence microplate reader at Ex/Em = 490/525 nm (cut off at 515 nm).
Calculators
Common stock solution preparation
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 138.75 µL | 693.751 µL | 1.388 mL | 6.938 mL | 13.875 mL |
5 mM | 27.75 µL | 138.75 µL | 277.5 µL | 1.388 mL | 2.775 mL |
10 mM | 13.875 µL | 69.375 µL | 138.75 µL | 693.751 µL | 1.388 mL |
Molarity calculator
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
References
Authors: James, Andrew D and Unthank, Katherine P and Jones, Isobel and Sajjaboontawee, Nattanan and Sizer, Rebecca E and Chawla, Sangeeta and Evans, Gareth J O and Brackenbury, William J
Journal: Physiological reports (2023): e15663
Authors: Morgan, Sherif H and Kader, Md Abdul and Lindberg, Sylvia
Journal: Plants (Basel, Switzerland) (2022)
Authors: Chung, Yu Jin and Park, Kyung Chan and Tokar, Sergiy and Eykyn, Thomas R and Fuller, William and Pavlovic, Davor and Swietach, Pawel and Shattock, Michael J
Journal: Cardiovascular research (2021): 2794-2806
Authors: Gerkau, Niklas J and Kafitz, Karl W and Rose, Christine R
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 187-202
Authors: Gerkau, Niklas J and Lerchundi, Rodrigo and Nelson, Joel S E and Lantermann, Marina and Meyer, Jan and Hirrlinger, Johannes and Rose, Christine R
Journal: The Journal of physiology (2019): 5687-5705
Authors: Moshrefi-Ravasdjani, Behrouz and Hammel, Evelyn L and Kafitz, Karl W and Rose, Christine R
Journal: Neurochemical research (2017): 2505-2518
Authors: Langer, Julia and Gerkau, Niklas J and Derouiche, Amin and Kleinhans, Christian and Moshrefi-Ravasdjani, Behrouz and Fredrich, Michaela and Kafitz, Karl W and Seifert, Gerald and Steinhäuser, Christian and Rose, Christine R
Journal: Glia (2017): 293-308
Authors: Yamazaki, Yui and Harada, Shinichi and Wada, Tetsuyuki and Hagiwara, Teruki and Yoshida, Shigeru and Tokuyama, Shogo
Journal: European journal of pharmacology (2017): 103-110
Authors: Gerkau, Niklas J and Rakers, Cordula and Petzold, Gabor C and Rose, Christine R
Journal: Journal of neuroscience research (2017): 2275-2285
Authors: Iamshanova, Oksana and Mariot, Pascal and Lehen'kyi, V'yacheslav and Prevarskaya, Natalia
Journal: European biophysics journal : EBJ (2016): 765-777
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