Tetracysteine REASH Reagent *2 mM DMSO Solution*

Tetracysteine REASH Reagent

2 mM DMSO Solution

REASH is a resorufin derivative, modified to contain two arsenic atoms at a set distance from each other. The biarsenical labeling technology works through the high-affinity interaction of arsenic for thiols. When REASH binds to tetracysteine sequences, its biarsenical group reacts rapidly with Cys-Cys moiety and the tag become highly fluorescent in red. The biarsenical labeling reagent REASH is one of the smallest expression tags for labeling a protein that contains a six-amino acid motif with a Cys-Cys-X1-X2-Cys-Cys amino acid sequence. The most commonly used tetracysteine is the six amino acid Cys-Cys-Pro-Gly-Cys-Cys sequence. As this sequence rarely appears in endogenous proteins, incorporating the sequence into target proteins generates a small but highly specific target for protein labeling. REASH generates a strong red fluorescent signal when binding to recombinant proteins containing the tetracysteine motif Cys-Cys-Pro-Gly-Cys-Cys. It can be used for monitoring protein localization, turnover and trafficking, receptor signaling and internalization.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22332	100 Tests	Price

References

View all 8 references: Citation Explorer

Discrete Virus Factories Form in the Cytoplasm of Cells Coinfected with Two Replication-Competent Tagged Reporter Birnaviruses That Subsequently Coalesce over Time.

Authors:

Campbell, Elle A and Reddy, Vishwanatha R A P and Gray, Alice G and Wells, Joanna and Simpson, Jennifer and Skinner, Michael A and Hawes, Philippa C and Broadbent, Andrew J

Journal:

Journal of virology (2020)

Tetracysteine-based fluorescent tags to study protein localization and trafficking in Plasmodium falciparum-infected erythrocytes.

Authors:

Crivat, Georgeta and Tokumasu, Fuyuki and Sa, Juliana Martha and Hwang, Jeeseong and Wellems, Thomas E

Journal:

PloS one (2011): e22975

Hairpin structure of a biarsenical-tetracysteine motif determined by NMR spectroscopy.

Authors:

Madani, Fatemeh and Lind, Jesper and Damberg, Peter and Adams, Stephen R and Tsien, Roger Y and Gräslund, Astrid O

Journal:

Journal of the American Chemical Society (2009): 4613-5

Helix A stabilization precedes amino-terminal lobe activation upon calcium binding to calmodulin.

Authors:

Chen, Baowei and Lowry, David F and Mayer, M Uljana and Squier, Thomas C

Journal:

Biochemistry (2008): 9220-6

Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins.

Authors:

Adams, Stephen R and Tsien, Roger Y

Journal:

Nature protocols (2008): 1527-34

Physical properties

Molecular weight	545.37
Solvent	DMSO

Spectral properties

Absorbance (nm)	570
Extinction coefficient (cm ^-1 M ^-1)	65000 ¹
Excitation (nm)	571
Emission (nm)	584
Quantum yield	0.75 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 3, 2025