Tetracysteine REASH Reagent *2 mM DMSO Solution*
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 545.37 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 570 |
Extinction coefficient (cm -1 M -1) | 650001 |
Excitation (nm) | 571 |
Emission (nm) | 584 |
Quantum yield | 0.751 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
Molecular weight 545.37 | Absorbance (nm) 570 | Extinction coefficient (cm -1 M -1) 650001 | Excitation (nm) 571 | Emission (nm) 584 | Quantum yield 0.751 |
REASH is a resorufin derivative, modified to contain two arsenic atoms at a set distance from each other. The biarsenical labeling technology works through the high-affinity interaction of arsenic for thiols. When REASH binds to tetracysteine sequences, its biarsenical group reacts rapidly with Cys-Cys moiety and the tag become highly fluorescent in red. The biarsenical labeling reagent REASH is one of the smallest expression tags for labeling a protein that contains a six-amino acid motif with a Cys-Cys-X1-X2-Cys-Cys amino acid sequence. The most commonly used tetracysteine is the six amino acid Cys-Cys-Pro-Gly-Cys-Cys sequence. As this sequence rarely appears in endogenous proteins, incorporating the sequence into target proteins generates a small but highly specific target for protein labeling. REASH generates a strong red fluorescent signal when binding to recombinant proteins containing the tetracysteine motif Cys-Cys-Pro-Gly-Cys-Cys. It can be used for monitoring protein localization, turnover and trafficking, receptor signaling and internalization.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tetracysteine REASH Reagent *2 mM DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 183.362 µL | 916.809 µL | 1.834 mL | 9.168 mL | 18.336 mL |
5 mM | 36.672 µL | 183.362 µL | 366.724 µL | 1.834 mL | 3.667 mL |
10 mM | 18.336 µL | 91.681 µL | 183.362 µL | 916.809 µL | 1.834 mL |
Molarity calculator
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Spectrum
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Spectral properties
Absorbance (nm) | 570 |
Extinction coefficient (cm -1 M -1) | 650001 |
Excitation (nm) | 571 |
Emission (nm) | 584 |
Quantum yield | 0.751 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Tetracysteine FLASH Reagent *2 mM DMSO Solution* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Tetracysteine REASH acetate *2 mM DMSO Solution* | 571 | 584 | 650001 | 0.751 | - | - |
References
View all 8 references: Citation Explorer
Discrete Virus Factories Form in the Cytoplasm of Cells Coinfected with Two Replication-Competent Tagged Reporter Birnaviruses That Subsequently Coalesce over Time.
Authors: Campbell, Elle A and Reddy, Vishwanatha R A P and Gray, Alice G and Wells, Joanna and Simpson, Jennifer and Skinner, Michael A and Hawes, Philippa C and Broadbent, Andrew J
Journal: Journal of virology (2020)
Authors: Campbell, Elle A and Reddy, Vishwanatha R A P and Gray, Alice G and Wells, Joanna and Simpson, Jennifer and Skinner, Michael A and Hawes, Philippa C and Broadbent, Andrew J
Journal: Journal of virology (2020)
Tetracysteine-based fluorescent tags to study protein localization and trafficking in Plasmodium falciparum-infected erythrocytes.
Authors: Crivat, Georgeta and Tokumasu, Fuyuki and Sa, Juliana Martha and Hwang, Jeeseong and Wellems, Thomas E
Journal: PloS one (2011): e22975
Authors: Crivat, Georgeta and Tokumasu, Fuyuki and Sa, Juliana Martha and Hwang, Jeeseong and Wellems, Thomas E
Journal: PloS one (2011): e22975
Hairpin structure of a biarsenical-tetracysteine motif determined by NMR spectroscopy.
Authors: Madani, Fatemeh and Lind, Jesper and Damberg, Peter and Adams, Stephen R and Tsien, Roger Y and Gräslund, Astrid O
Journal: Journal of the American Chemical Society (2009): 4613-5
Authors: Madani, Fatemeh and Lind, Jesper and Damberg, Peter and Adams, Stephen R and Tsien, Roger Y and Gräslund, Astrid O
Journal: Journal of the American Chemical Society (2009): 4613-5
Helix A stabilization precedes amino-terminal lobe activation upon calcium binding to calmodulin.
Authors: Chen, Baowei and Lowry, David F and Mayer, M Uljana and Squier, Thomas C
Journal: Biochemistry (2008): 9220-6
Authors: Chen, Baowei and Lowry, David F and Mayer, M Uljana and Squier, Thomas C
Journal: Biochemistry (2008): 9220-6
Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins.
Authors: Adams, Stephen R and Tsien, Roger Y
Journal: Nature protocols (2008): 1527-34
Authors: Adams, Stephen R and Tsien, Roger Y
Journal: Nature protocols (2008): 1527-34
New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications.
Authors: Adams, Stephen R and Campbell, Robert E and Gross, Larry A and Martin, Brent R and Walkup, Grant K and Yao, Yong and Llopis, Juan and Tsien, Roger Y
Journal: Journal of the American Chemical Society (2002): 6063-76
Authors: Adams, Stephen R and Campbell, Robert E and Gross, Larry A and Martin, Brent R and Walkup, Grant K and Yao, Yong and Llopis, Juan and Tsien, Roger Y
Journal: Journal of the American Chemical Society (2002): 6063-76
Tetracysteine genetic tags complexed with biarsenical ligands as a tool for investigating gap junction structure and dynamics.
Authors: Sosinsky, Gina E and Gaietta, Guido M and Hand, Galen and Deerinck, Thomas J and Han, Areum and Mackey, Mason and Adams, Stephen R and Bouwer, James and Tsien, Roger Y and Ellisman, Mark H
Journal: Cell communication & adhesion : 181-6
Authors: Sosinsky, Gina E and Gaietta, Guido M and Hand, Galen and Deerinck, Thomas J and Han, Areum and Mackey, Mason and Adams, Stephen R and Bouwer, James and Tsien, Roger Y and Ellisman, Mark H
Journal: Cell communication & adhesion : 181-6
Identification of an orthogonal peptide binding motif for biarsenical multiuse affinity probes.
Authors: Chen, Baowei and Cao, Haishi and Yan, Ping and Mayer, M Uljana and Squier, Thomas C
Journal: Bioconjugate chemistry : 1259-65
Authors: Chen, Baowei and Cao, Haishi and Yan, Ping and Mayer, M Uljana and Squier, Thomas C
Journal: Bioconjugate chemistry : 1259-65