Tetracysteine REASH Reagent *2 mM DMSO Solution*

Tetracysteine REASH Reagent

2 mM DMSO Solution

Tetracysteine REASH Reagent is a red fluorescent biarsenical labeling reagent for detecting recombinant proteins containing a tetracysteine motif.

Tetracysteine tag labeling: Binds recombinant proteins containing a Cys-Cys-X1-X2-Cys-Cys tetracysteine motif
Small expression tag: Uses a compact six-amino acid recognition sequence for site-specific labeling of engineered proteins
Red fluorescence activation: Becomes highly fluorescent after binding to the tetracysteine sequence through biarsenical-thiol interaction
Protein tracking applications: Suitable for monitoring protein localization, turnover, trafficking, receptor signaling, and internalization

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22332	100 Tests	Price

Overview

Tetracysteine REASH Reagent is a resorufin-derived biarsenical probe modified with two arsenic atoms positioned to interact with tetracysteine-containing protein tags. The biarsenical labeling technology is based on the high-affinity interaction between arsenic groups and thiols.

Tetracysteine REASH Reagent binds recombinant proteins engineered with a tetracysteine sequence, typically a Cys-Cys-X1-X2-Cys-Cys motif. When REASH binds to the tetracysteine sequence, its biarsenical group reacts rapidly with the Cys-Cys moiety, generating a strong red fluorescent signal. This compact tag-based labeling approach is useful for studying protein localization, turnover, trafficking, receptor signaling, and internalization.

References

View all 8 references: Citation Explorer

Discrete Virus Factories Form in the Cytoplasm of Cells Coinfected with Two Replication-Competent Tagged Reporter Birnaviruses That Subsequently Coalesce over Time.

Authors:

Campbell, Elle A and Reddy, Vishwanatha R A P and Gray, Alice G and Wells, Joanna and Simpson, Jennifer and Skinner, Michael A and Hawes, Philippa C and Broadbent, Andrew J

Journal:

Journal of virology (2020)

Tetracysteine-based fluorescent tags to study protein localization and trafficking in Plasmodium falciparum-infected erythrocytes.

Authors:

Crivat, Georgeta and Tokumasu, Fuyuki and Sa, Juliana Martha and Hwang, Jeeseong and Wellems, Thomas E

Journal:

PloS one (2011): e22975

Hairpin structure of a biarsenical-tetracysteine motif determined by NMR spectroscopy.

Authors:

Madani, Fatemeh and Lind, Jesper and Damberg, Peter and Adams, Stephen R and Tsien, Roger Y and Gräslund, Astrid O

Journal:

Journal of the American Chemical Society (2009): 4613-5

Helix A stabilization precedes amino-terminal lobe activation upon calcium binding to calmodulin.

Authors:

Chen, Baowei and Lowry, David F and Mayer, M Uljana and Squier, Thomas C

Journal:

Biochemistry (2008): 9220-6

Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins.

Authors:

Adams, Stephen R and Tsien, Roger Y

Journal:

Nature protocols (2008): 1527-34

Physical properties

Molecular weight	545.37
Solvent	DMSO

Spectral properties

Absorbance (nm)	570
Extinction coefficient (cm ^-1 M ^-1)	65000 ¹
Excitation (nm)	571
Emission (nm)	584
Quantum yield	0.75 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy3 filter set
Emission	Cy3 filter set
Recommended plate	Black wall/clear bottom

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Page updated on August 29, 2026