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Tide Quencher™ 4WS-DBCO [TQ4WS-DBCO]

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Additional ordering information
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1158.49
Spectral properties
Absorbance (nm)605
Correction Factor (260 nm)0.149
Correction Factor (280 nm)0.136
Extinction coefficient (cm -1 M -1)900001
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Related products
Tide Quencher™ 2WS acid [TQ2WS acid]
Tide Quencher™ 2WS succinimidyl ester [TQ2WS, SE]
Tide Quencher™ 2WS maleimide [TQ2WS maleimide]
Tide Quencher™ 4 CPG [TQ4 CPG] *500 Å*
Tide Quencher™ 4 CPG [TQ4 CPG] *1000 Å*
Tide Quencher™ 4WS succinimidyl ester [TQ4WS SE]
Tide Quencher™ 5WS acid [TQ5WS acid]
Tide Quencher™ 5WS amine [TQ5WS amine]
Tide Quencher™ 5 CPG [TQ5 CPG] *500 Å*
Tide Quencher™ 5 CPG [TQ5 CPG] *1000 Å*
Tide Quencher™ 5WS maleimide [TQ5WS maleimide]
Tide Quencher™ 5WS succinimidyl ester [TQ5WS SE]
Tide Quencher™ 5WS alkyne [TQ5WS alkyne]
Tide Quencher™ 6WS acid [TQ6WS acid]
Tide Quencher™ 6WS amine [TQ6WS amine]
Tide Quencher™ 6WS maleimide [TQ6WS maleimide]
Tide Quencher™ 6WS succinimidyl ester [TQ6WS SE]
Tide Quencher™ 6WS azide [TQ6WS azide]
Tide Quencher™ 6WS alkyne [TQ6WS alkyne]
Tide Quencher™ 7WS acid [TQ7WS acid]
Tide Quencher™ 7WS amine [TQ7WS amine]
Tide Quencher™ 7WS maleimide [TQ7WS maleimide]
Tide Quencher™ 7WS succinimidyl ester [TQ7WS SE]
Tide Quencher™ 7WS alkyne [TQ7WS alkyne]
Tide Quencher™ 1 azide [TQ1 azide]
Tide Quencher™ 1 alkyne [TQ1 alkyne]
Tide Quencher™ 1 acid [TQ1 acid]
Tide Quencher™ 1 amine [TQ1 amine]
Tide Quencher™ 1 CPG [TQ1 CPG] *500 Å*
Tide Quencher™ 1 CPG [TQ1 CPG] *1000 Å*
Tide Quencher™ 1 maleimide [TQ1 maleimide]
Tide Quencher™ 1 phosphoramidite [TQ1 phosphoramidite]
Tide Quencher™ 1 succinimidyl ester [TQ1 SE]
Tide Quencher™ 2 acid [TQ2 acid]
Tide Quencher™ 2 amine [TQ2 amine]
Tide Quencher™ 2 CPG [TQ2 CPG] *500 Å*
Tide Quencher™ 2 CPG [TQ2 CPG] *1000 Å*
Tide Quencher™ 2 phosphoramidite [TQ2 phosphoramidite]
Tide Quencher™ 2 succinimidyl ester [TQ2 SE]
Tide Quencher™ 2 azide [TQ2 azide]
Tide Quencher™ 2 alkyne [TQ2 alkyne]
Tide Quencher™ 3 acid [TQ3 acid]
Tide Quencher™ 3 amine [TQ3 amine]
Tide Quencher™ 3 CPG [TQ3 CPG] *500 Å*
Tide Quencher™ 3 CPG [TQ3 CPG] *1000 Å*
Tide Quencher™ 3 maleimide [TQ3 maleimide]
Tide Quencher™ 3WS acid [TQ3WS acid]
Tide Quencher™ 3 phosphoramidite [TQ3 phosphoramidite]
Tide Quencher™ 3WS succinimidyl ester [TQ3WS SE]
Tide Quencher™ 3 succinimidyl ester [TQ3 SE]
Tide Quencher™ 3 azide [TQ3 azide]
Tide Quencher™ 3 alkyne [TQ3 alkyne]
Tide Quencher™ 2WS alkyne [TQ2WS alkyne]
Tide Quencher™ 5WS azide [TQ5WS azide]
Tide Quencher™ 7WS azide [TQ7WS azide]
Show More (45)


Molecular weight
Absorbance (nm)
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
TQ4WS is designed to be a superior quencher to ROX, TF4, iFluor® 594, Alexa Fluor® 594 and Texas Red®. TQ4WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ4WS-DBCO product is reactive to azides under copper-free conditions, and useful for click chemistry. DBCO is probably the most common alkyne among the strain promoted alkyne-azide cycloaddition (SPAAC), which is also termed as the Cu-free click reaction. Cyclooctynes and azides exclusively and efficiently react with each other while remain inert to naturally occurring functional groups such as amines. SPAAC enables labeling a wide variety of biomolecules without any auxiliary reagents in an aqueous and otherwise complex chemical environment through the formation of a stable triazole. DBCO (dibenzocyclooctynes) compounds comprise a class of reagents that possesses reasonably fast kinetics and good stability in aqueous buffers. Within physiological temperature and pH ranges, the DBCO group will not react with amines or hydroxyls that are naturally present in many biomolecules. Additionally, reaction of the DBCO group with the azide group is significantly faster than with sulfhydryl groups (–SH, thiol).


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 4WS-DBCO [TQ4WS-DBCO] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM86.319 µL431.596 µL863.193 µL4.316 mL8.632 mL
5 mM17.264 µL86.319 µL172.639 µL863.193 µL1.726 mL
10 mM8.632 µL43.16 µL86.319 µL431.596 µL863.193 µL

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Spectral properties

Absorbance (nm)605
Correction Factor (260 nm)0.149
Correction Factor (280 nm)0.136
Extinction coefficient (cm -1 M -1)900001

Product Family

NameExtinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Tide Quencher™ 4WS acid [TQ4WS acid]9000010.1490.136
Tide Quencher™ 4WS amine [TQ4WS amine]9000010.1490.136
Tide Quencher™ 4WS maleimide [TQ4WS maleimide]9000010.1490.136
Tide Quencher™ 4WS azide [TQ4WS azide]9000010.1490.136
Tide Quencher™ 4WS alkyne [TQ4WS alkyne]9000010.1490.136



View all 50 references: Citation Explorer
pH-Triggered Copper-Free Click Reaction-Mediated Micelle Aggregation for Enhanced Tumor Retention and Elevated Immuno-Chemotherapy against Melanoma.
Authors: Deng, Miao and Guo, Rong and Zang, Shuya and Rao, Jingdong and Li, Mengmeng and Tang, Xian and Xia, Chunyu and Li, Man and Zhang, Zhirong and He, Qin
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Cytosolic protein delivery via metabolic glycoengineering and bioorthogonal click reactions.
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Cancer cell-targeted cisplatin prodrug delivery in vivo via metabolic labeling and bioorthogonal click reaction.
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Interventional nuclear medicine: "click" chemistry as an in vivo targeting strategy for imaging microspheres and bacteria.
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Development of a Cancer Vaccine Using In Vivo Click-Chemistry-Mediated Active Lymph Node Accumulation for Improved Immunotherapy.
Authors: Qin, Hao and Zhao, Ruifang and Qin, Yuting and Zhu, Jin and Chen, Long and Di, Chunzhi and Han, Xuexiang and Cheng, Keman and Zhang, Yinlong and Zhao, Ying and Shi, Jian and Anderson, Gregory J and Zhao, Yuliang and Nie, Guangjun
Journal: Advanced materials (Deerfield Beach, Fla.) (2021): e2006007
Covalent Cell Surface Conjugation of Nanoparticles by a Combination of Metabolic Labeling and Click Chemistry.
Authors: Lamoot, Alexander and Uvyn, Annemiek and Kasmi, Sabah and De Geest, Bruno G
Journal: Angewandte Chemie (International ed. in English) (2021): 6320-6325
Understanding selectivity of metabolic labelling and click-targeting in multicellular environments as a route to tissue selective drug delivery.
Authors: Tan, Angel and Liu, Qingtao and Septiadi, Dedy and Chu, Shuiling and Liu, Tianqing and Richards, Sarah-Jane and Rothen-Rutishauser, Barbara and Petri-Fink, Alke and Gibson, Matthew I and Boyd, Ben J
Journal: Journal of materials chemistry. B (2021): 5365-5373
A copper-free and enzyme-free click chemistry-mediated single quantum dot nanosensor for accurate detection of microRNAs in cancer cells and tissues.
Authors: Wang, Zi-Yue and Li, Dong-Ling and Tian, Xiaorui and Zhang, Chun-Yang
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CEBA: A new heterobifunctional reagent for plasmid DNA functionalization by click chemistry.
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