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Tide Quencher™ 4WS-DBCO [TQ4WS-DBCO]

TQ4WS is designed to be a superior quencher to ROX, TF4, iFluor® 594, Alexa Fluor® 594 and Texas Red®. TQ4WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ4WS-DBCO product is reactive to azides under copper-free conditions, and useful for click chemistry. DBCO is probably the most common alkyne among the strain promoted alkyne-azide cycloaddition (SPAAC), which is also termed as the Cu-free click reaction. Cyclooctynes and azides exclusively and efficiently react with each other while remain inert to naturally occurring functional groups such as amines. SPAAC enables labeling a wide variety of biomolecules without any auxiliary reagents in an aqueous and otherwise complex chemical environment through the formation of a stable triazole. DBCO (dibenzocyclooctynes) compounds comprise a class of reagents that possesses reasonably fast kinetics and good stability in aqueous buffers. Within physiological temperature and pH ranges, the DBCO group will not react with amines or hydroxyls that are naturally present in many biomolecules. Additionally, reaction of the DBCO group with the azide group is significantly faster than with sulfhydryl groups (–SH, thiol).

Spectrum

Product family

NameExtinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Tide Quencher™ 4WS acid [TQ4WS acid]9000010.1490.136
Tide Quencher™ 4WS amine [TQ4WS amine]9000010.1490.136
Tide Quencher™ 4WS maleimide [TQ4WS maleimide]9000010.1490.136
Tide Quencher™ 4WS azide [TQ4WS azide]9000010.1490.136
Tide Quencher™ 4WS alkyne [TQ4WS alkyne]9000010.1490.136

References

View all 50 references: Citation Explorer
pH-Triggered Copper-Free Click Reaction-Mediated Micelle Aggregation for Enhanced Tumor Retention and Elevated Immuno-Chemotherapy against Melanoma.
Authors: Deng, Miao and Guo, Rong and Zang, Shuya and Rao, Jingdong and Li, Mengmeng and Tang, Xian and Xia, Chunyu and Li, Man and Zhang, Zhirong and He, Qin
Journal: ACS applied materials & interfaces (2021): 18033-18046
Cytosolic protein delivery via metabolic glycoengineering and bioorthogonal click reactions.
Authors: Zhao, Ziyin and Zhang, Zhimin and Duan, Shanzhou and Liu, Xun and Zhou, Renxiang and Hou, Mengying and Sang, Yonghua and Zhu, Rongying and Yin, Lichen
Journal: Biomaterials science (2021): 4639-4647
Cancer cell-targeted cisplatin prodrug delivery in vivo via metabolic labeling and bioorthogonal click reaction.
Authors: Liu, Xun and Wu, Fan and Cai, Kaimin and Zhao, Ziyin and Zhang, Zhimin and Chen, Yongbing and Liu, Yong and Cheng, Jianjun and Yin, Lichen
Journal: Biomaterials science (2021): 1301-1312
Interventional nuclear medicine: "click" chemistry as an in vivo targeting strategy for imaging microspheres and bacteria.
Authors: Welling, M M and Duszenko, N and van Willigen, D M and Hensbergen, A W and Buckle, T and Rietbergen, D D D and Roestenberg, M and van Leeuwen, F W B
Journal: Biomaterials science (2021): 1683-1690
In vivo vocal fold augmentation using an injectable polyethylene glycol hydrogel based on click chemistry.
Authors: Kwon, Soonmin and Choi, Hyunsu and Park, Changhee and Choi, Sangkee and Kim, Eunha and Kim, Sung Won and Kim, Choung-Soo and Koo, Heebeom
Journal: Biomaterials science (2021): 108-115
Page updated on October 8, 2024

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Catalog Number2070
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Physical properties

Molecular weight

1158.49

Solvent

DMSO

Spectral properties

Absorbance (nm)

605

Correction Factor (260 nm)

0.149

Correction Factor (280 nm)

0.136

Extinction coefficient (cm -1 M -1)

900001

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501