Tide Quencher™ 4WS-DBCO [TQ4WS-DBCO]
Ordering information
Price | |
Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1158.49 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 605 |
Correction Factor (260 nm) | 0.149 |
Correction Factor (280 nm) | 0.136 |
Extinction coefficient (cm -1 M -1) | 900001 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
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See also: Tide Quencher Dyes
Molecular weight 1158.49 | Absorbance (nm) 605 | Correction Factor (260 nm) 0.149 | Correction Factor (280 nm) 0.136 | Extinction coefficient (cm -1 M -1) 900001 |
TQ4WS is designed to be a superior quencher to ROX, TF4, iFluor® 594, Alexa Fluor® 594 and Texas Red®. TQ4WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ4WS-DBCO product is reactive to azides under copper-free conditions, and useful for click chemistry. DBCO is probably the most common alkyne among the strain promoted alkyne-azide cycloaddition (SPAAC), which is also termed as the Cu-free click reaction. Cyclooctynes and azides exclusively and efficiently react with each other while remain inert to naturally occurring functional groups such as amines. SPAAC enables labeling a wide variety of biomolecules without any auxiliary reagents in an aqueous and otherwise complex chemical environment through the formation of a stable triazole. DBCO (dibenzocyclooctynes) compounds comprise a class of reagents that possesses reasonably fast kinetics and good stability in aqueous buffers. Within physiological temperature and pH ranges, the DBCO group will not react with amines or hydroxyls that are naturally present in many biomolecules. Additionally, reaction of the DBCO group with the azide group is significantly faster than with sulfhydryl groups (–SH, thiol).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 4WS-DBCO [TQ4WS-DBCO] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 86.319 µL | 431.596 µL | 863.193 µL | 4.316 mL | 8.632 mL |
5 mM | 17.264 µL | 86.319 µL | 172.639 µL | 863.193 µL | 1.726 mL |
10 mM | 8.632 µL | 43.16 µL | 86.319 µL | 431.596 µL | 863.193 µL |
Molarity calculator
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Spectrum
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Spectral properties
Absorbance (nm) | 605 |
Correction Factor (260 nm) | 0.149 |
Correction Factor (280 nm) | 0.136 |
Extinction coefficient (cm -1 M -1) | 900001 |
Product Family
Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
Tide Quencher™ 4WS acid [TQ4WS acid] | 900001 | 0.149 | 0.136 |
Tide Quencher™ 4WS amine [TQ4WS amine] | 900001 | 0.149 | 0.136 |
Tide Quencher™ 4WS maleimide [TQ4WS maleimide] | 900001 | 0.149 | 0.136 |
Tide Quencher™ 4WS azide [TQ4WS azide] | 900001 | 0.149 | 0.136 |
Tide Quencher™ 4WS alkyne [TQ4WS alkyne] | 900001 | 0.149 | 0.136 |
References
View all 50 references: Citation Explorer
pH-Triggered Copper-Free Click Reaction-Mediated Micelle Aggregation for Enhanced Tumor Retention and Elevated Immuno-Chemotherapy against Melanoma.
Authors: Deng, Miao and Guo, Rong and Zang, Shuya and Rao, Jingdong and Li, Mengmeng and Tang, Xian and Xia, Chunyu and Li, Man and Zhang, Zhirong and He, Qin
Journal: ACS applied materials & interfaces (2021): 18033-18046
Authors: Deng, Miao and Guo, Rong and Zang, Shuya and Rao, Jingdong and Li, Mengmeng and Tang, Xian and Xia, Chunyu and Li, Man and Zhang, Zhirong and He, Qin
Journal: ACS applied materials & interfaces (2021): 18033-18046
Cytosolic protein delivery via metabolic glycoengineering and bioorthogonal click reactions.
Authors: Zhao, Ziyin and Zhang, Zhimin and Duan, Shanzhou and Liu, Xun and Zhou, Renxiang and Hou, Mengying and Sang, Yonghua and Zhu, Rongying and Yin, Lichen
Journal: Biomaterials science (2021): 4639-4647
Authors: Zhao, Ziyin and Zhang, Zhimin and Duan, Shanzhou and Liu, Xun and Zhou, Renxiang and Hou, Mengying and Sang, Yonghua and Zhu, Rongying and Yin, Lichen
Journal: Biomaterials science (2021): 4639-4647
Cancer cell-targeted cisplatin prodrug delivery in vivo via metabolic labeling and bioorthogonal click reaction.
Authors: Liu, Xun and Wu, Fan and Cai, Kaimin and Zhao, Ziyin and Zhang, Zhimin and Chen, Yongbing and Liu, Yong and Cheng, Jianjun and Yin, Lichen
Journal: Biomaterials science (2021): 1301-1312
Authors: Liu, Xun and Wu, Fan and Cai, Kaimin and Zhao, Ziyin and Zhang, Zhimin and Chen, Yongbing and Liu, Yong and Cheng, Jianjun and Yin, Lichen
Journal: Biomaterials science (2021): 1301-1312
Interventional nuclear medicine: "click" chemistry as an in vivo targeting strategy for imaging microspheres and bacteria.
Authors: Welling, M M and Duszenko, N and van Willigen, D M and Hensbergen, A W and Buckle, T and Rietbergen, D D D and Roestenberg, M and van Leeuwen, F W B
Journal: Biomaterials science (2021): 1683-1690
Authors: Welling, M M and Duszenko, N and van Willigen, D M and Hensbergen, A W and Buckle, T and Rietbergen, D D D and Roestenberg, M and van Leeuwen, F W B
Journal: Biomaterials science (2021): 1683-1690
In vivo vocal fold augmentation using an injectable polyethylene glycol hydrogel based on click chemistry.
Authors: Kwon, Soonmin and Choi, Hyunsu and Park, Changhee and Choi, Sangkee and Kim, Eunha and Kim, Sung Won and Kim, Choung-Soo and Koo, Heebeom
Journal: Biomaterials science (2021): 108-115
Authors: Kwon, Soonmin and Choi, Hyunsu and Park, Changhee and Choi, Sangkee and Kim, Eunha and Kim, Sung Won and Kim, Choung-Soo and Koo, Heebeom
Journal: Biomaterials science (2021): 108-115
Development of a Cancer Vaccine Using In Vivo Click-Chemistry-Mediated Active Lymph Node Accumulation for Improved Immunotherapy.
Authors: Qin, Hao and Zhao, Ruifang and Qin, Yuting and Zhu, Jin and Chen, Long and Di, Chunzhi and Han, Xuexiang and Cheng, Keman and Zhang, Yinlong and Zhao, Ying and Shi, Jian and Anderson, Gregory J and Zhao, Yuliang and Nie, Guangjun
Journal: Advanced materials (Deerfield Beach, Fla.) (2021): e2006007
Authors: Qin, Hao and Zhao, Ruifang and Qin, Yuting and Zhu, Jin and Chen, Long and Di, Chunzhi and Han, Xuexiang and Cheng, Keman and Zhang, Yinlong and Zhao, Ying and Shi, Jian and Anderson, Gregory J and Zhao, Yuliang and Nie, Guangjun
Journal: Advanced materials (Deerfield Beach, Fla.) (2021): e2006007
Covalent Cell Surface Conjugation of Nanoparticles by a Combination of Metabolic Labeling and Click Chemistry.
Authors: Lamoot, Alexander and Uvyn, Annemiek and Kasmi, Sabah and De Geest, Bruno G
Journal: Angewandte Chemie (International ed. in English) (2021): 6320-6325
Authors: Lamoot, Alexander and Uvyn, Annemiek and Kasmi, Sabah and De Geest, Bruno G
Journal: Angewandte Chemie (International ed. in English) (2021): 6320-6325
Understanding selectivity of metabolic labelling and click-targeting in multicellular environments as a route to tissue selective drug delivery.
Authors: Tan, Angel and Liu, Qingtao and Septiadi, Dedy and Chu, Shuiling and Liu, Tianqing and Richards, Sarah-Jane and Rothen-Rutishauser, Barbara and Petri-Fink, Alke and Gibson, Matthew I and Boyd, Ben J
Journal: Journal of materials chemistry. B (2021): 5365-5373
Authors: Tan, Angel and Liu, Qingtao and Septiadi, Dedy and Chu, Shuiling and Liu, Tianqing and Richards, Sarah-Jane and Rothen-Rutishauser, Barbara and Petri-Fink, Alke and Gibson, Matthew I and Boyd, Ben J
Journal: Journal of materials chemistry. B (2021): 5365-5373
A copper-free and enzyme-free click chemistry-mediated single quantum dot nanosensor for accurate detection of microRNAs in cancer cells and tissues.
Authors: Wang, Zi-Yue and Li, Dong-Ling and Tian, Xiaorui and Zhang, Chun-Yang
Journal: Chemical science (2021): 10426-10435
Authors: Wang, Zi-Yue and Li, Dong-Ling and Tian, Xiaorui and Zhang, Chun-Yang
Journal: Chemical science (2021): 10426-10435
CEBA: A new heterobifunctional reagent for plasmid DNA functionalization by click chemistry.
Authors: Gao, Haifei and Gonçalves, Cristine and Maze, Delphine and Pichon, Chantal and Midoux, Patrick
Journal: International journal of pharmaceutics (2021): 120566
Authors: Gao, Haifei and Gonçalves, Cristine and Maze, Delphine and Pichon, Chantal and Midoux, Patrick
Journal: International journal of pharmaceutics (2021): 120566