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trFluor™ Eu DBCO *europium complex*

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Physical properties
Molecular weight1765.72
Spectral properties
Correction Factor (260 nm)0.911
Correction Factor (280 nm)0.777
Extinction coefficient (cm -1 M -1)21000
Excitation (nm)298
Emission (nm)617
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed. The use of long-lived fluorophores combined with time-resolved detection (a delay between excitation and emission detection) minimizes prompt fluorescence interferences. Our trFluor™ Eu probes enable time-resolved fluorometry (TRF) for the assays that require high sensitivity. These trFluor™ Eu probes have large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, our trFluor™ Eu probes have relatively high stability, high emission yield and ability to be linked to biomolecules. Moreover, our trFluor™ Eu probes are insensitive to fluorescence quenching when conjugated to biological polymers such as antibodies. trFluor™ Eu DBCO *europium complex* can be readily clicked to molecules containing an azido group under copper-free conditions.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of trFluor™ Eu DBCO *europium complex* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM56.634 µL283.171 µL566.341 µL2.832 mL5.663 mL
5 mM11.327 µL56.634 µL113.268 µL566.341 µL1.133 mL
10 mM5.663 µL28.317 µL56.634 µL283.171 µL566.341 µL

Molarity calculator

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Spectral properties

Correction Factor (260 nm)0.911
Correction Factor (280 nm)0.777
Extinction coefficient (cm -1 M -1)21000
Excitation (nm)298
Emission (nm)617

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
trFluor™ Eu maleimide *europium complex*298617210000.9110.777



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