TRITC–dextran conjugate (average MW = ~150K)

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Physical properties

Molecular weight	N/A
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.27
Correction Factor (280 nm)	0.34
Extinction coefficient (cm ^-1 M ^-1)	100000
Excitation (nm)	544
Emission (nm)	570

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

N/A

Correction Factor (260 nm)

0.27

Correction Factor (280 nm)

0.34

Extinction coefficient (cm ^-1 M ^-1)

100000

Excitation (nm)

544

Emission (nm)

570

TRITC-labeled dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. It is also used in microcirculation and cell permeability research utilizing microfluorimetry, and possible in perfusion studies in animals. TRITC-dextran has also been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to TRITC-dextran. TRITC-dextran of 150 kDa may be used to help assess the integrity of the blood brain barrier (BBB) and to perform fluorescence microlymphography. It may also be used as a microcirculation vascular flux tracer.

Spectrum

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Spectral properties

Correction Factor (260 nm)	0.27
Correction Factor (280 nm)	0.34
Extinction coefficient (cm ^-1 M ^-1)	100000
Excitation (nm)	544
Emission (nm)	570

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)	Correction Factor (260 nm)
FITC–dextran conjugate (average MW = ~150K)	491	516	73000	0.35	-
TRITC–dextran conjugate (average MW = ~20K)	544	570	100000	0.34	0.27
TRITC–dextran conjugate (average MW = ~70K)	544	570	100000	0.34	0.27

Images

Figure 1. Chemical structure for TRITC–dextran conjugate (average MW = ~150K).

Citations

View all 7 citations: Citation Explorer

SIRB, sans iron oxide rhodamine B, a novel cross-linked dextran nanoparticle, labels human neuroprogenitor and SH-SY5Y neuroblastoma cells and serves as a USPIO cell labeling control
Authors: Shen, W. B., Vaccaro, D. E., Fishman, P. S., Groman, E. V., Yarowsky, P.
Journal: Contrast Media Mol Imaging (2016): 222-8

In vivo retrograde labeling of neurons in the zebrafish embryo or larva with rhodamine dextran
Authors: Volkmann, K., Koster, R. W.
Journal: CSH Protoc (2007): pdb prot4832

Tracing of axonal connectivities in a combined slice preparation of rat brains--a study by rhodamine-dextran-amine-application in the lateral nucleus of the amygdala
Authors: von Bohlen und Halbach, O., Albrecht, D.
Journal: J Neurosci Methods (1998): 169-75

Multiple anterograde tracing, combining Phaseolus vulgaris leucoagglutinin with rhodamine- and biotin-conjugated dextran amine
Authors: Dolleman-Van der Weel, M. J., Wouterlood, F. G., Witter, M. P.
Journal: J Neurosci Methods (1994): 9-21

Tracing of axonal connections by rhodamine-dextran-amine in the rat hippocampal-entorhinal cortex slice preparation
Authors: Boulton, C. L., von Haebler, D., Heinemann, U.
Journal: Hippocampus (1992): 99-106

Labeling of developing vascular endothelium after injections of rhodamine-dextran into blastomeres of Xenopus laevis
Authors: Rovainen, C. M.
Journal: J Exp Zool (1991): 209-21

In vivo anterograde and retrograde axonal transport of the fluorescent rhodamine-dextran-amine, Fluoro-Ruby, within the CNS
Authors: Schmued, L., Kyriakidis, K., Heimer, L.
Journal: Brain Res (1990): 127-34