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TWO-PRO™ 1
equivalent to TO-PRO®-1; 5 mM DMSO Solution; CAS 157199-59-2
TWO-PRO™-1 is chemically equivalent to TO-PRO®-1 (TO-PRO® is the trademark of Invitrogen). TWO-PRO™-1 is a carbocyanine dimer with green fluorescence similar to FITC. It is cell-impermeant and easily distinguished from Cy5 and rhodamines as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. TWO-PRO™-1 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Red™ LCS1 dye and cell-impermeant TWO-PRO™-1 can be used to assess cell viability. TWO-PRO™-1 and Nuclear Red™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.
Chemical structure for TWO-PRO™ 1  [equivalent to TO-PRO®-1] *5 mM DMSO Solution* *CAS 157199-59-2*
Chemical structure for TWO-PRO™ 1  [equivalent to TO-PRO®-1] *5 mM DMSO Solution* *CAS 157199-59-2*
CatalogSize
Price
Quantity
175710.2 ml
Price
 
Physical properties

Molecular weight645.38
SolventDMSO
Spectral properties

Extinction coefficient (cm -1 M -1)
62800
1
Excitation (nm)515
Emission (nm)531
Quantum yield
0.25
1
Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
CAS157199-59-2
Instrument settings

Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
Instrument specification(s)FITC filter set
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Page updated on October 12, 2025