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TWO-PRO™ 3
equivalent to TO-PRO®-3; 5 mM DMSO Solution; CAS 157199-63-8
TWO-PRO™-3 is chemically equivalent to TO-PRO®-3 (TO-PRO® is the trademark of Invitrogen). TWO-PRO™-3 is a carbocyanine dimer with far-red fluorescence similar to Cy® 5 dyes. It is cell-impermeant and easily distinguished from fluorescein and rhodamine as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. TWO-PRO™-3 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Green™ LCS1 dye and cell-impermeant TWO-PRO™-3 can be used to assess cell viability. TWO-PRO™-3 and Nuclear Green™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.
Chemical structure for TWO-PRO™ 3 [equivalent to TO-PRO®-3] *5 mM DMSO Solution* *CAS 157199-63-8*
Chemical structure for TWO-PRO™ 3 [equivalent to TO-PRO®-3] *5 mM DMSO Solution* *CAS 157199-63-8*
CatalogSize
Price
Quantity
175720.2 ml
Price
 
Physical properties

Molecular weight671.42
SolventDMSO
Spectral properties

Extinction coefficient (cm -1 M -1)
102000
1
Excitation (nm)642
Emission (nm)657
Quantum yield
0.11
1
Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
Instrument settings

Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom
Instrument specification(s)Cy5 filter set
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Page updated on October 9, 2025