Name: TWO-PRO™ 3 [equivalent to TO-PRO®-3] *5 mM DMSO Solution* *CAS 157199-63-8*
Brand: AAT Bioquest
SKU: 17572
Price: 232 USD
Availability: InStock

TWO-PRO™ 3

equivalent to TO-PRO®-3; 5 mM DMSO Solution; CAS 157199-63-8

TWO-PRO™-3 is chemically equivalent to TO-PRO®-3 (TO-PRO® is the trademark of Invitrogen). TWO-PRO™-3 is a carbocyanine dimer with far-red fluorescence similar to Cy® 5 dyes. It is cell-impermeant and easily distinguished from fluorescein and rhodamine as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. TWO-PRO™-3 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Green™ LCS1 dye and cell-impermeant TWO-PRO™-3 can be used to assess cell viability. TWO-PRO™-3 and Nuclear Green™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.

Protocol

SDS

Catalog	Size	Price	Quantity
17572	0.2 ml	Price

Citations

View all 2 citations: Citation Explorer

Synergistic Activity and Mechanism of Sanguinarine with Polymyxin B against Gram-Negative Bacterial Infections

Authors:

Qiao, Luyao and Zhang, Yu and Chen, Ying and Chi, Xiangyin and Ding, Jinwen and Zhang, Hongjuan and Han, Yanxing and Zhang, Bo and Jiang, Jiandong and Lin, Yuan

Journal:

Pharmaceutics (2024): 70

A lysosomal regulatory circuit essential for the development and function of microglia

Authors:

Iyer, Harini and Shen, Kimberle and Meireles, Ana M and Talbot, William S

Journal:

Science advances (2022): eabp8321

References

View all 42 references: Citation Explorer

Daptomycin exerts rapid bactericidal activity against Bacillus anthracis without disrupting membrane integrity

Authors:

Xing YH, Wang W, Dai SQ, Liu TY, Tan JJ, Qu GL, Li YX, Ling Y, Liu G, Fu XQ, Chen HP.

Journal:

Acta Pharmacol Sin (2014): 211

Quantification of Candida albicans by flow cytometry using TO-PRO((R))-3 iodide as a single-stain viability dye

Authors:

Kerstens M, Boulet G, Pintelon I, Hellings M, Voeten L, Delputte P, Maes L, Cos P.

Journal:

J Microbiol Methods (2013): 189

A silicon cell cycle in a bacterial model of calcium phosphate mineralogenesis

Authors:

Linton KM, Tapping CR, Adams DG, Carter RD, Shore RC, Aaron JE.

Journal:

Micron (2013): 419

Pulsed electromagnetic field affects intrinsic and endoplasmatic reticulum apoptosis induction pathways in MonoMac6 cell line culture

Authors:

Kaszuba-Zwoinska J, Chorobik P, Juszczak K, Zaraska W, Thor PJ.

Journal:

J Physiol Pharmacol (2012): 537

Determination of the drug-DNA binding modes using fluorescence-based assays

Authors:

Williams AK, Dasilva SC, Bhatta A, Rawal B, Liu M, Korobkova EA.

Journal:

Anal Biochem (2012): 66

Physical properties

Molecular weight	671.42
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	102000 ¹
Excitation (nm)	642
Emission (nm)	657
Quantum yield	0.11 ¹

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Instrument settings

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Cy5 filter set

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Viability & Proliferation
Brochure: Nucleic Acid Detection Probes & Assay Kits

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Page updated on October 31, 2025