logo
AAT Bioquest

VIC phosphoramidite

VIC is a fluorescent dye that was originally developed by Applied Biosystems (now, Thermofisher). VIC emits in the green-yellow part of the visible spectrum. It is used to fluorescently label oligonucleotides at the 5’-end (for use as probes in a variety of real-time PCR, hybridization, and fluorescence-based genetic analysis applications). VIC phosphoramidite is the most convenient building block to incorporate VIC tag into an oligonucleotide.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy3 phosphoramidite55556915000010.1510.070.073
Cy5 phosphoramidite65167025000010.271, 0.420.020.03

References

View all 5 references: Citation Explorer
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Simultaneous detection of the main black aspergilli responsible for ochratoxin A (OTA) contamination in grapes by multiplex real-time polymerase chain reaction.
Authors: Selma, M V and Martínez-Culebras, P V and Elizaquível, P and Aznar, R
Journal: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment (2009): 180-8
Multiplex real-time PCR for the detection and differentiation of equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4).
Authors: Diallo, Ibrahim S and Hewitson, Glen and Wright, Lucia L and Kelly, Mark A and Rodwell, Barry J and Corney, Bruce G
Journal: Veterinary microbiology (2007): 93-103
Detection and quantitation of akabane and aino viruses by multiplex real-time reverse-transcriptase PCR.
Authors: Stram, Yehuda and Kuznetzova, Larisa and Guini, Merisol and Rogel, Arie and Meirom, Ruth and Chai, Dalia and Yadin, Hagai and Brenner, Jackob
Journal: Journal of virological methods (2004): 147-54
Structure of the cytochrome c oxidase complex of rat liver. 2. Topological orientation of polypeptides in the membrane as studied by proteolytic digestion and immunoblotting.
Authors: Jarausch, J and Kadenbach, B
Journal: European journal of biochemistry (1985): 219-25
Page updated on July 26, 2023

Ordering information

Price
Unit size
50 umoles
100 umoles
1 g
Catalog Number
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

1023.38

Solvent

MeCN

Spectral properties

Excitation (nm)

526

Emission (nm)

543

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

1414265-81-8