VIC phosphoramidite
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1023.38 |
Solvent | MeCN |
Spectral properties
Excitation (nm) | 526 |
Emission (nm) | 543 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Purification & Analysis, PCR Detection of Viral DNA/RNA
CAS 1414265-81-8 | Molecular weight 1023.38 | Excitation (nm) 526 | Emission (nm) 543 |
VIC is a fluorescent dye that was originally developed by Applied Biosystems (now, Thermofisher). VIC emits in the green-yellow part of the visible spectrum. It is used to fluorescently label oligonucleotides at the 5’-end (for use as probes in a variety of real-time PCR, hybridization, and fluorescence-based genetic analysis applications). VIC phosphoramidite is the most convenient building block to incorporate VIC tag into an oligonucleotide.
Calculators
Common stock solution preparation
Table 1. Volume of MeCN needed to reconstitute specific mass of VIC phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 97.715 µL | 488.577 µL | 977.154 µL | 4.886 mL | 9.772 mL |
5 mM | 19.543 µL | 97.715 µL | 195.431 µL | 977.154 µL | 1.954 mL |
10 mM | 9.772 µL | 48.858 µL | 97.715 µL | 488.577 µL | 977.154 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Cy3 phosphoramidite | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
Cy5 phosphoramidite | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
References
View all 5 references: Citation Explorer
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Simultaneous detection of the main black aspergilli responsible for ochratoxin A (OTA) contamination in grapes by multiplex real-time polymerase chain reaction.
Authors: Selma, M V and Martínez-Culebras, P V and Elizaquível, P and Aznar, R
Journal: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment (2009): 180-8
Authors: Selma, M V and Martínez-Culebras, P V and Elizaquível, P and Aznar, R
Journal: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment (2009): 180-8
Multiplex real-time PCR for the detection and differentiation of equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4).
Authors: Diallo, Ibrahim S and Hewitson, Glen and Wright, Lucia L and Kelly, Mark A and Rodwell, Barry J and Corney, Bruce G
Journal: Veterinary microbiology (2007): 93-103
Authors: Diallo, Ibrahim S and Hewitson, Glen and Wright, Lucia L and Kelly, Mark A and Rodwell, Barry J and Corney, Bruce G
Journal: Veterinary microbiology (2007): 93-103
Detection and quantitation of akabane and aino viruses by multiplex real-time reverse-transcriptase PCR.
Authors: Stram, Yehuda and Kuznetzova, Larisa and Guini, Merisol and Rogel, Arie and Meirom, Ruth and Chai, Dalia and Yadin, Hagai and Brenner, Jackob
Journal: Journal of virological methods (2004): 147-54
Authors: Stram, Yehuda and Kuznetzova, Larisa and Guini, Merisol and Rogel, Arie and Meirom, Ruth and Chai, Dalia and Yadin, Hagai and Brenner, Jackob
Journal: Journal of virological methods (2004): 147-54
Structure of the cytochrome c oxidase complex of rat liver. 2. Topological orientation of polypeptides in the membrane as studied by proteolytic digestion and immunoblotting.
Authors: Jarausch, J and Kadenbach, B
Journal: European journal of biochemistry (1985): 219-25
Authors: Jarausch, J and Kadenbach, B
Journal: European journal of biochemistry (1985): 219-25
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?