XFD488 C5 Maleimide Same Structure to Alexa Fluor™ 488 C5 Maleimide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	720.66
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.30
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	490
Emission (nm)	520
Quantum yield	0.92¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

720.66

Correction Factor (260 nm)

0.30

Correction Factor (280 nm)

0.11

Extinction coefficient (cm ^-1 M ^-1)

71000

Excitation (nm)

490

Emission (nm)

520

Quantum yield

0.92¹

XFD488 C5 Maleimide is the same molecule to the Alexa Fluor® 488 C5 Maleimide (Alexa Fluor® is the trademark of ThermoFisher). It is a bright green-fluorescent dye optimal for use with the 488 nm Argon laser. XFD488 dye is water soluble and pH-insensitive from pH 4 to pH 10. The C5 maleimide of XFD488 is the most convenient thiol-reactive form for conjugating this dye to a protein or antibody.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	138.762 µL	693.808 µL	1.388 mL	6.938 mL	13.876 mL
5 mM	27.752 µL	138.762 µL	277.523 µL	1.388 mL	2.775 mL
10 mM	13.876 µL	69.381 µL	138.762 µL	693.808 µL	1.388 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.30
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	490
Emission (nm)	520
Quantum yield	0.92¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD350 C5 Maleimide Same Structure to Alexa Fluor™ 350 C5 Maleimide	303	439	19000	-	0.25	0.19
XFD532 C5 Maleimide Same Structure to Alexa Fluor™ 532 C5 Maleimide	317	553	81000	0.61¹	0.24	0.09
XFD594 C5 Maleimide Same Structure to Alexa Fluor™ 594 C5 Maleimide	375	618	90000	0.66¹	0.43	0.56
EDANS C5 maleimide	336	455	5900	-	-	0.107

Citations

View all 1 citations: Citation Explorer

Kinetic quantitative analysis reveals the suppression of Sup35NM amyloid fibril nucleation by liquid-liquid phase separation.
Authors: Fukuyama, Mao and Nishinami, Suguru and Maruyama, Yoko and Ozawa, Taiki and Tomita, Shunsuke and Ohhashi, Yumiko and Kasuya, Motohiro and Gen, Masao and Chatani, Eri and Shiraki, Kentaro and others,
Journal: (2022)

References

View all 39 references: Citation Explorer

Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22

Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep

Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601

In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12

A mouse monoclonal antibody against Alexa Fluor 647
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20

Volume labeling with Alexa Fluor dyes and surface functionalization of highly sensitive fluorescent silica (SiO2) nanoparticles
Authors: Wang, W.; Nallathamby, P. D.; Foster, C. M.; Morrell-Falvey, J. L.; Mortensen, N. P.; Doktycz, M. J.; Gu, B.; Retterer, S. T.
Journal: Nanoscale (2013): 10369-75

Green tea catechins quench the fluorescence of bacteria-conjugated Alexa fluor dyes
Authors: Zhao, L.; Li, W.; Zhu, S.; Tsai, S.; Li, J.; Tracey, K. J.; Wang, P.; Fan, S.; Sama, A. E.; Wang, H.
Journal: Inflamm Allergy Drug Targets (2013): 308-14

Fluorescence of Alexa fluor dye tracks protein folding
Authors: Lindhoud, S.; Westphal, A. H.; Visser, A. J.; Borst, J. W.; van Mierlo, C. P.
Journal: PLoS One (2012): e46838

Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)