XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 720.66 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.30 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 71000 |
Excitation (nm) | 499 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
See also: Antibody and Protein Labeling
Molecular weight 720.66 | Correction Factor (260 nm) 0.30 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 71000 | Excitation (nm) 499 | Emission (nm) 520 | Quantum yield 0.921 |
XFD488 C5 Maleimide is the same molecule to the Alexa Fluor® 488 C5 Maleimide (Alexa Fluor® is the trademark of ThermoFisher). It is a bright green-fluorescent dye optimal for use with the 488 nm Argon laser. XFD488 dye is water soluble and pH-insensitive from pH 4 to pH 10. The C5 maleimide of XFD488 is the most convenient thiol-reactive form for conjugating this dye to a protein or antibody.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 138.762 µL | 693.808 µL | 1.388 mL | 6.938 mL | 13.876 mL |
5 mM | 27.752 µL | 138.762 µL | 277.523 µL | 1.388 mL | 2.775 mL |
10 mM | 13.876 µL | 69.381 µL | 138.762 µL | 693.808 µL | 1.388 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.30 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 71000 |
Excitation (nm) | 499 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD350 C5 Maleimide *Same Structure to Alexa Fluor™ 350 C5 Maleimide* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
XFD532 C5 Maleimide *Same Structure to Alexa Fluor™ 532 C5 Maleimide* | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
XFD594 C5 Maleimide *Same Structure to Alexa Fluor™ 594 C5 Maleimide* | 590 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
EDANS C5 maleimide | 336 | 455 | 5900 | - | - | 0.107 |
Images

Figure 1. Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide, or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
Citations
View all 1 citations: Citation Explorer
Kinetic quantitative analysis reveals the suppression of Sup35NM amyloid fibril nucleation by liquid-liquid phase separation.
Authors: Fukuyama, Mao and Nishinami, Suguru and Maruyama, Yoko and Ozawa, Taiki and Tomita, Shunsuke and Ohhashi, Yumiko and Kasuya, Motohiro and Gen, Masao and Chatani, Eri and Shiraki, Kentaro and others,
Journal: (2022)
Authors: Fukuyama, Mao and Nishinami, Suguru and Maruyama, Yoko and Ozawa, Taiki and Tomita, Shunsuke and Ohhashi, Yumiko and Kasuya, Motohiro and Gen, Masao and Chatani, Eri and Shiraki, Kentaro and others,
Journal: (2022)
References
View all 39 references: Citation Explorer
Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
A mouse monoclonal antibody against Alexa Fluor 647
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20
Volume labeling with Alexa Fluor dyes and surface functionalization of highly sensitive fluorescent silica (SiO2) nanoparticles
Authors: Wang, W.; Nallathamby, P. D.; Foster, C. M.; Morrell-Falvey, J. L.; Mortensen, N. P.; Doktycz, M. J.; Gu, B.; Retterer, S. T.
Journal: Nanoscale (2013): 10369-75
Authors: Wang, W.; Nallathamby, P. D.; Foster, C. M.; Morrell-Falvey, J. L.; Mortensen, N. P.; Doktycz, M. J.; Gu, B.; Retterer, S. T.
Journal: Nanoscale (2013): 10369-75
Green tea catechins quench the fluorescence of bacteria-conjugated Alexa fluor dyes
Authors: Zhao, L.; Li, W.; Zhu, S.; Tsai, S.; Li, J.; Tracey, K. J.; Wang, P.; Fan, S.; Sama, A. E.; Wang, H.
Journal: Inflamm Allergy Drug Targets (2013): 308-14
Authors: Zhao, L.; Li, W.; Zhu, S.; Tsai, S.; Li, J.; Tracey, K. J.; Wang, P.; Fan, S.; Sama, A. E.; Wang, H.
Journal: Inflamm Allergy Drug Targets (2013): 308-14
Fluorescence of Alexa fluor dye tracks protein folding
Authors: Lindhoud, S.; Westphal, A. H.; Visser, A. J.; Borst, J. W.; van Mierlo, C. P.
Journal: PLoS One (2012): e46838
Authors: Lindhoud, S.; Westphal, A. H.; Visser, A. J.; Borst, J. W.; van Mierlo, C. P.
Journal: PLoS One (2012): e46838
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
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