XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 643.40 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.30 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 71000 |
Excitation (nm) | 490 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
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See also: Amine Reactive Dyes and Probes for Conjugation, Antibodies and Proteomics, Antibody and Protein Labeling, Bioconjugation, Chemical Reagents, Dyes by Functional Group
Molecular weight 643.40 | Correction Factor (260 nm) 0.30 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 71000 | Excitation (nm) 490 | Emission (nm) 520 | Quantum yield 0.921 |
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). XFD488 NHS Ester (Succinimidyl Ester) has the same molecule as Alexa Fluor® 488 NHS Ester (#A20000 and A20100 from ThermoFisher). It is a bright green-fluorescent dye optimal for use with the 488 nm Argon laser. XFD488 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD488 NHS ester (or succinimidyl ester) is the most convenient amine-reactive form for conjugating this dye to a protein or antibody. Although the Alexa Fluor® 488 NHS Ester is one of the most common green fluorescent labeling dyes, its purity has been quite challenging. ThermoFisher provides it with purity of ~50-60% while a few other vendors offer it at even lower purities. Among all the impurities, we noted that the Bis-NHS ester of XFD488 is quite detrimental since it could give a non-fluorescent product. This high purity XFD488 NHS ester is free of the detrimental XFD488 Bis-NHS ester impurity with the minimal purity of >75%.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 155.424 µL | 777.122 µL | 1.554 mL | 7.771 mL | 15.542 mL |
5 mM | 31.085 µL | 155.424 µL | 310.849 µL | 1.554 mL | 3.108 mL |
10 mM | 15.542 µL | 77.712 µL | 155.424 µL | 777.122 µL | 1.554 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.30 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 71000 |
Excitation (nm) | 490 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Citations
View all 1 citations: Citation Explorer
An ULK1/2-PXN mechanotransduction complex suppresses breast cancer cell migration
Authors: Liang, Peigang and Zhang, Jiaqi and Wu, Yuchen and Zheng, Shanyuan and Xu, Zhaopeng and Yang, Shuo and Wang, Jinfang and Ma, Suibin and Xiao, Li and Wu, Tianhui and others,
Journal: bioRxiv (2023): 2023--02
Authors: Liang, Peigang and Zhang, Jiaqi and Wu, Yuchen and Zheng, Shanyuan and Xu, Zhaopeng and Yang, Shuo and Wang, Jinfang and Ma, Suibin and Xiao, Li and Wu, Tianhui and others,
Journal: bioRxiv (2023): 2023--02
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)