Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488*

Ordering information
Price ()
Catalog Number1810
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight643.40
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)499
Emission (nm)520
Quantum yield0.921
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Related products
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide*
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne*
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*
XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide*
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester*
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester*
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*
XFD350 C5 Maleimide *Same Structure to Alexa Fluor™ 350 C5 Maleimide*
XFD532 C5 Maleimide *Same Structure to Alexa Fluor™ 532 C5 Maleimide*
XFD594 C5 Maleimide *Same Structure to Alexa Fluor™ 594 C5 Maleimide*
XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine*
XFD350 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*
XFD488 tyramide reagent *Same Structure to Alexa Fluor™ 488 tyramide*
XFD546 tyramide reagent *Same Structure to Alexa Fluor™ 546 tyramide*
XFD594 tyramide reagent *Same Structure to Alexa Fluor™ 594 tyramide*
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester*
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester*
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester*
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester*
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester*
XFD647 maleimide *C2, XFD647 Same Structure to Alexa Fluor™ 647*
XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester*
XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester*
XFD350 tyramide reagent *Same Structure to Alexa Fluor™ 350 tyramide*
XFD568 tyramide reagent *Same Structure to Alexa Fluor™ 568 tyramide*
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*
XFD™488-dUTP *1 mM in Tris Buffer (pH 7.5)*
XFD514 acid
XFD514 NHS Ester *Same Structure to Alexa Fluor™ 514 NHS Ester*
XFD514 tyramide
XFD532 tyramide
XFD647 Azide
XFD647 Alkyne
XFD488 TCO
Show More (53)

OverviewpdfSDSpdfProtocol


Molecular weight
643.40
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)
499
Emission (nm)
520
Quantum yield
0.921
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). XFD488 NHS Ester (Succinimidyl Ester) has the same molecule as Alexa Fluor® 488 NHS Ester (#A20000 and A20100 from ThermoFisher). It is a bright green-fluorescent dye optimal for use with the 488 nm Argon laser. XFD488 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD488 NHS ester (or succinimidyl ester) is the most convenient amine-reactive form for conjugating this dye to a protein or antibody. Although the Alexa Fluor® 488 NHS Ester is one of the most common green fluorescent labeling dyes, its purity has been quite challenging. ThermoFisher provides it with purity of ~50-60% while a few other vendors offer it at even lower purities. Among all the impurities, we noted that the Bis-NHS ester of XFD488 is quite detrimental since it could give a non-fluorescent product. This high purity XFD488 NHS ester is free of the detrimental XFD488 Bis-NHS ester impurity with the minimal purity of >75%.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM155.424 µL777.122 µL1.554 mL7.771 mL15.542 mL
5 mM31.085 µL155.424 µL310.849 µL1.554 mL3.108 mL
10 mM15.542 µL77.712 µL155.424 µL777.122 µL1.554 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)73000
Excitation (nm)499
Emission (nm)520
Quantum yield0.921