XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488*
| Overview |  SDSProtocol |
See also: Amine Reactive Dyes and Probes for Conjugation, Bioconjugation, Chemical Reagents, Dyes by Functional Group
Molecular weight 643.40 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 499 | Emission (nm) 520 | Quantum yield 0.921 |
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). XFD488 NHS Ester (Succinimidyl Ester) has the same molecule as Alexa Fluor® 488 NHS Ester (#A20000 and A20100 from ThermoFisher). It is a bright green-fluorescent dye optimal for use with the 488 nm Argon laser. XFD488 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD488 NHS ester (or succinimidyl ester) is the most convenient amine-reactive form for conjugating this dye to a protein or antibody. Although the Alexa Fluor® 488 NHS Ester is one of the most common green fluorescent labeling dyes, its purity has been quite challenging. ThermoFisher provides it with purity of ~50-60% while a few other vendors offer it at even lower purities. Among all the impurities, we noted that the Bis-NHS ester of XFD488 is quite detrimental since it could give a non-fluorescent product. This high purity XFD488 NHS ester is free of the detrimental XFD488 Bis-NHS ester impurity with the minimal purity of >75%.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 155.424 µL | 777.122 µL | 1.554 mL | 7.771 mL | 15.542 mL |
| 5 mM | 31.085 µL | 155.424 µL | 310.849 µL | 1.554 mL | 3.108 mL |
| 10 mM | 15.542 µL | 77.712 µL | 155.424 µL | 777.122 µL | 1.554 mL |
Molarity calculator
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Spectrum
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Spectral properties
| Extinction coefficient (cm -1 M -1) | 73000 |
| Excitation (nm) | 499 |
| Emission (nm) | 520 |
| Quantum yield | 0.921 |
Application notes
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FITC (Fluorescein isothiocyanate)
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