XFD532 amine
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 896.83 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.24 |
| Correction Factor (280 nm) | 0.09 |
| Extinction coefficient (cm -1 M -1) | 81000 |
| Excitation (nm) | 534 |
| Emission (nm) | 553 |
| Quantum yield | 0.611 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
| Overview |  SDSProtocol |
Molecular weight 896.83 | Correction Factor (260 nm) 0.24 | Correction Factor (280 nm) 0.09 | Extinction coefficient (cm -1 M -1) 81000 | Excitation (nm) 534 | Emission (nm) 553 | Quantum yield 0.611 |
XFD532 amine is the same molecule to the Alexa Fluor® 532 amine (Alexa Fluor® is the trademark of ThermoFisher). It is a bright yellow-fluorescent dye optimal for use with the frequency-doubled Nd:YAG laser line. XFD532 dye is water soluble and pH-insensitive from pH 4 to pH 10. The amine of XFD532 is a versatile carbonyl-reactive form for conjugating this fluorophore to peptides and other biological molecules.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD532 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 111.504 µL | 557.519 µL | 1.115 mL | 5.575 mL | 11.15 mL |
| 5 mM | 22.301 µL | 111.504 µL | 223.008 µL | 1.115 mL | 2.23 mL |
| 10 mM | 11.15 µL | 55.752 µL | 111.504 µL | 557.519 µL | 1.115 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.24 |
| Correction Factor (280 nm) | 0.09 |
| Extinction coefficient (cm -1 M -1) | 81000 |
| Excitation (nm) | 534 |
| Emission (nm) | 553 |
| Quantum yield | 0.611 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 375 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
| XFD532 acid *Same Structure to Alexa Fluor™ 532 acid* | 317 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| XFD532 tyramide | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| XFD555 amine | 553 | 568 | 155000 | 0.11 | 0.08 | 0.08 |
| XFD568 amine | 579 | 603 | 88000 | 0.691 | 0.45 | 0.46 |
| XFD647 amine | 650 | 671 | 270000 | 0.331 | - | 0.03 |
| XFD750 amine | 752 | 776 | 290000 | 0.121 | - | 0.04 |
| ICG amine | 789 | 814 | - | - | - | 0.076 |
Images
Figure 1. XFD532 amine is the same molecule to the Alexa Fluor® 532 amine. It is a bright yellow-fluorescent dye optimal for use with the frequency-doubled Nd:YAG laser line. XFD532 dye is water soluble and pH-insensitive from pH 4 to pH 10.
References
View all 40 references: Citation Explorer
Visualization of preimplantation uterine fluid absorption in mice using Alexa Fluor™ 488 hydrazide.
Authors: Li, Yuehuan and Martin, Taylor Elijah and Hancock, Jonathan Matthew and Li, Rong and Viswanathan, Suvitha and Lydon, John P and Zheng, Yi and Ye, Xiaoqin
Journal: Biology of reproduction (2022)
Authors: Li, Yuehuan and Martin, Taylor Elijah and Hancock, Jonathan Matthew and Li, Rong and Viswanathan, Suvitha and Lydon, John P and Zheng, Yi and Ye, Xiaoqin
Journal: Biology of reproduction (2022)
Alexa Fluor 488-conjugated cholera toxin subunit B optimally labels neurons 3-7 days after injection into the rat gastrocnemius muscle.
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320
Click-iT ® Plus OPP Alexa Fluor ® Protein Synthesis Assay in Embryonic Cells.
Authors: Li, Yan and Ji, Xu and Chang, Lu and Tang, Jianan and Hua, Min-Min and Liu, Jing and O'Neill, Christopher and Huang, Xuefeng and Jin, Xingliang
Journal: Bio-protocol (2022): e4441
Authors: Li, Yan and Ji, Xu and Chang, Lu and Tang, Jianan and Hua, Min-Min and Liu, Jing and O'Neill, Christopher and Huang, Xuefeng and Jin, Xingliang
Journal: Bio-protocol (2022): e4441
Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.
Authors: Ahishali, Bulent and Kaya, Mehmet
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 87-103
Authors: Ahishali, Bulent and Kaya, Mehmet
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 87-103
Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845
Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)
Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021): 1546
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021): 1546
Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568.
Authors: Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós
Journal: Journal of fluorescence (2020): 437-443
Authors: Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós
Journal: Journal of fluorescence (2020): 437-443
Photo-isomerization of the Cyanine Dye Alexa-Fluor 647 (AF-647) in the Context of dSTORM Super-Resolution Microscopy.
Authors: Karlsson, Joshua K G and Laude, Alex and Hall, Michael J and Harriman, Anthony
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2019): 14983-14998
Authors: Karlsson, Joshua K G and Laude, Alex and Hall, Michael J and Harriman, Anthony
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2019): 14983-14998
Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy.
Authors: Fan, Sanjun and Webb, James E A and Yang, Ying and Nieves, Daniel J and Gonçales, Vinicius R and Tran, Jason and Hilzenrat, Geva and Kahram, Mohaddeseh and Tilley, Richard D and Gaus, Katharina and Gooding, J Justin
Journal: Angewandte Chemie (International ed. in English) (2019): 14495-14498
Authors: Fan, Sanjun and Webb, James E A and Yang, Ying and Nieves, Daniel J and Gonçales, Vinicius R and Tran, Jason and Hilzenrat, Geva and Kahram, Mohaddeseh and Tilley, Richard D and Gaus, Katharina and Gooding, J Justin
Journal: Angewandte Chemie (International ed. in English) (2019): 14495-14498