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XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*

Ordering information
Price ()
Catalog Number16892
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight52000
SolventWater
Spectral properties
Absorbance (nm)592
Correction Factor (260 nm)0.43
Correction Factor (280 nm)0.56
Extinction coefficient (cm -1 M -1)92000
Excitation (nm)590
Emission (nm)618
Quantum yield0.661
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide*
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester*
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XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*
XFD350 C5 Maleimide *Same Structure to Alexa Fluor™ 350 C5 Maleimide*
XFD532 C5 Maleimide *Same Structure to Alexa Fluor™ 532 C5 Maleimide*
XFD594 C5 Maleimide *Same Structure to Alexa Fluor™ 594 C5 Maleimide*
XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine*
XFD350 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
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XFD594 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*
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XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*
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XFD546 tyramide reagent *Same Structure to Alexa Fluor™ 546 tyramide*
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XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester*
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XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester*
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester*
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester*
XFD647 maleimide *C2, XFD647 Same Structure to Alexa Fluor™ 647*
XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester*
XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester*
XFD350 tyramide reagent *Same Structure to Alexa Fluor™ 350 tyramide*
XFD568 tyramide reagent *Same Structure to Alexa Fluor™ 568 tyramide*
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*
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XFD514 tyramide
XFD532 tyramide
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XFD647 Alkyne
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Show More (86)

OverviewpdfSDSpdfProtocol


Molecular weight
52000
Absorbance (nm)
592
Correction Factor (260 nm)
0.43
Correction Factor (280 nm)
0.56
Extinction coefficient (cm -1 M -1)
92000
Excitation (nm)
590
Emission (nm)
618
Quantum yield
0.661
XFD594 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher). The conjugates of streptavidin are commonly used together with a biotin conjugate for specific detection of a variety of proteins, protein motifs, nucleic acids, and other biomolecules in western blots, flow cytometry, imaging and microscopy, and microplate assays. XFD594-streptavidin conjugate is equivalent to Alexa Fluor® 594 streptavidin. The conjugate is comprised of a biotin-binding streptavidin covalently attached to XFD594 dye.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM1.923 µL9.615 µL19.231 µL96.154 µL192.308 µL
5 mM384.615 nL1.923 µL3.846 µL19.231 µL38.462 µL
10 mM192.308 nL961.538 nL1.923 µL9.615 µL19.231 µL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)592
Correction Factor (260 nm)0.43
Correction Factor (280 nm)0.56
Extinction coefficient (cm -1 M -1)92000
Excitation (nm)590
Emission (nm)618
Quantum yield0.661

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*34344119000-0.19
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*499520730000.9210.11
RPE-streptavidin conjugate56657419600000.82-
APC-streptavidin conjugate651660700000--
PerCP-streptavidin conjugate477678350000--
FITC-streptavidin conjugate491516730000.920.254
Cy3®-streptavidin conjugate55556915000010.1510.073
Cy5®-streptavidin conjugate65167025000010.271, 0.420.03
Cy7®-streptavidin conjugate7567792500000.30.036
HRP-streptavidin conjugate-----
AP-streptavidin conjugate [Streptavidin-alkaline phosphatase conjugate]-----
Show More (12)

Citations


View all 4 citations: Citation Explorer
Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.
Authors: Zhao, Jingkun and Ou, Baochi and Feng, Hao and Wang, Puxiongzhi and Yin, Shuai and Zhu, Congcong and Wang, Shenjie and Chen, Chun and Zheng, Minhua and Zong, Yaping and others, undefined
Journal: Oncotarget (2017)
Cadherin-12 enhances proliferation in colorectal cancer cells and increases progression by promoting EMT
Authors: Ma, Junjun and Zhao, Jingkun and Lu, Jun and Wang, Puxiongzhi and Feng, Hao and Zong, Yaping and Ou, Baochi and Zheng, Minhua and Lu, Aiguo
Journal: Tumor Biology (2016): 1--12
Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury
Authors: Shi, W and Huang, CJ and Xu, XD and Jin, GH and Huang, RQ and Huang, JF and Chen, YN and Ju, SQ and Wang, Y and Shi, YW and others, undefined
Journal: Acta Biomaterialia (2016): 247--261
The migration and differentiation of hUC-MSCsCXCR4/GFP encapsulated in BDNF/chitosan scaffolds for brain tissue engineering
Authors: Huang, Chuanjun and Zhao, Longxiang and Gu, Jun and Nie, Dekang and Chen, Yinan and Zuo, Hao and Huan, Wei and Shi, Jinlong and Chen, Jian and Shi, Wei
Journal: Biomedical Materials (2016): 035004

References


View all 191 references: Citation Explorer
Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668
Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728
Falsely elevated tacrolimus levels caused by immunoassay interference secondary to beta-galactosidase antibodies in an infected liver transplant recipient
Authors: Knorr JP, Grewal KS, Balasubramanian M, Young N, Zaki R, Khanmoradi K, Araya V, Ortiz J.
Journal: Pharmacotherapy (2010): 954
Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency
Authors: Buckova A., undefined
Journal: Epidemiol Mikrobiol Imunol (2010): 133
Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis
Authors: Boonrod K, Roth B, Leong Ngar S, Krczal G.
Journal: Anal Biochem (2010): 124
Nanosilver-doped DNA polyion complex membrane for electrochemical immunoassay of carcinoembryonic antigen using nanogold-labeled secondary antibodies
Authors: Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.
Journal: Anal Chim Acta (2010): 126
Three siblings with self-resolving congenital hyperthyrotropinaemia secondary to thyrotropin receptor blocking antibodies
Authors: Azzopardi P, Forrester M, Ehtisham S.
Journal: J Paediatr Child Health (2010): 439
Double-blotting: a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures
Authors: Lasne F., undefined
Journal: Methods Mol Biol (2009): 213
Parkinsonism/dystonia syndrome secondary to multiple sclerosis with anti-basal-ganglia antibodies
Authors: Delgado S, Baez S, Singer C, Sengun C, Sheremata WA, Papapetropoulos S.
Journal: Mov Disord (2009): 309
Neutralizing antibodies and secondary therapy failure after treatment with botulinum toxin type A: much ado about nothing
Authors: Lange O, Bigalke H, Dengler R, Wegner F, deGroot M, Wohlfarth K.
Journal: Clin Neuropharmacol (2009): 213