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Xite™ Red beta-D-galactopyranoside
Xite™ Red beta-D-galactopyranoside provides a simple and sensitive tool to detect beta-galactosidase (β-gal) activity. Compared to the existing red beta-galactosidase substrates (e.g., the commonly used resorufin beta-D-galactopyranoside), it has much better cell permeability. Xite™ Red beta-D-galactopyranoside provides a simple and sensitive tool to detect beta-galactosidase activity. Xite™ Red beta-D-galactopyranoside might be used as a simple tool for measuring cellular senescence in cells since β-gal has been identified as a reliable marker for cellular senescence. Xite™ Red beta-D-galactopyranoside enters readily cells where it gets cleaved by β-gal, producing Xite™ Red, a strongly fluorescent product. The strongly fluorescent Xite™ Red is well retained in cells, making it easy to be detected with a flow cytometer and fluorescence microscope. In addition, Xite™ Red beta-D-galactopyranoside is fixable. The red fluorescence generated by Xite™ Red beta-D-galactopyranoside can be readily combined with other color fluorescent probes such as DAPI or GFP for multicolor fluorescence analysis.
Expression of β-gal was measured with Xite™ Red beta-D-galactopyranoside. 9L-LacZ cells (cells that overexpressed β-gal) were incubated with Xite™ Red beta-D-galactopyranoside for 30 mins at 37 °C. The signal was acquired with PE channel using a NovoCyte Flow Cytometer (ACEA Biosciences).
Expression of β-gal was measured with Xite™ Red beta-D-galactopyranoside. 9L-LacZ cells (cells that overexpressed β-gal) were incubated with Xite™ Red beta-D-galactopyranoside for 30 mins at 37 °C. The signal was acquired with PE channel using a NovoCyte Flow Cytometer (ACEA Biosciences).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
140351 mg
Price
 
Physical properties
Molecular weight591.63
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission575/26 nm filter
Instrument specification(s)PE channel
Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 23, 2025