Xite™ Red beta-D-galactopyranoside

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	591.63
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Overview SDS Protocol

Platform

Flow cytometer

Excitation	488 nm laser
Emission	575/26 nm filter
Instrument specification(s)	PE channel

Fluorescence microscope

Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

Example protocol

AT A GLANCE

Protocol summary

Treat samples as desired.
Prepare and add Xite™ Red beta-D-galactopyranoside working solution to samples
Incubate samples at 37 °C for 15 to 45 minutes
Monitor the fluorescence intensity using flow cytometer with 575/26 nm filter (PE channel) or using fluorescence microscopy with Cy3/TRITC filter set

PREPARATION OF STOCK SOLUTIONS

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

Xite™ Red beta-D-galactopyranoside stock solution

Add appropriate amount of DMSO into Xite™ Red beta-D-galactopyranoside to make 2-5 mM Xite™ Red beta-D-galactopyranoside stock solution. Note: Store the unused Xite™ Red beta-D-galactopyranoside stock solution at -20 °C in single use aliquots.

PREPARATION OF WORKING SOLUTION

Xite™ Red beta-D-galactopyranoside working solution

Prepare 1-20 µM of Xite™ Red beta-D-galactopyranoside working solution in buffer of your choice. Note: Xite™ Red beta-D-galactopyranoside working solution should be used promptly. Note: The concentration of the Xite™ Red beta-D-galactopyranoside should be optimized for different cell types and conditions.

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used as a guideline and should be optimized according to the needs.

Treat your samples as desired.
Remove the treatment and wash cells with buffer of your choice such as DPBS. Note: For selectively tracking β-Gal in live cells, cells can be treated with Bafilomycin A1 for blocking endogenous β-Gal. Optimum concentration of Bafilomycin A1 may vary on type of cells.
Add Xite™ Red beta-D-galactopyranoside working solution for 15-45 minutes and incubate the samples at 37 °C incubator. Note: Optimal time for incubation needs to be determined experimentally.
Remove the working solution and wash cells with buffer of your choice.
Resuspend the cells in buffer of your choice and monitor the fluorescence intensity with flow cytometer using 575/26 nm filter (PE channel) or fluorescence microscope with Cy3/TRITC filter set.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Xite™ Red beta-D-galactopyranoside to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	169.025 µL	845.123 µL	1.69 mL	8.451 mL	16.902 mL
5 mM	33.805 µL	169.025 µL	338.049 µL	1.69 mL	3.38 mL
10 mM	16.902 µL	84.512 µL	169.025 µL	845.123 µL	1.69 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Expression of β-gal was measured with Xite™ Red beta-D-galactopyranoside. 9L-LacZ cells (cells that overexpressed β-gal) were incubated with Xite™ Red beta-D-galactopyranoside for 30 mins at 37 °C. The signal was acquired with PE channel using a NovoCyte Flow Cytometer (ACEA Biosciences).

References

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Acridinium Benzoates for Ratiometric Fluorescence Imaging.
Authors: Wen, Min and Wang, Xijing and Wang, Ting and Sun, Yan and Fan, Mengting and Li, Min and Zhu, Junru and Zhang, Dazhi and Cui, Xiaoyan and Shan, Yongkui
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 3247-3251

Fluorescence Signal Amplification by Using β-Galactosidase for Flow Cytometry; Advantages of an Endogenous Activity-Free Enzyme.
Authors: Nobori, Takanobu and Kawamura, Masumi and Yoshida, Ryosuke and Joichi, Taisei and Kamino, Kenta and Kishimura, Akihiro and Baba, Eishi and Mori, Takeshi and Katayama, Yoshiki
Journal: Analytical chemistry (2020): 3069-3076

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Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2019): 1447-1460

Macrotheranostic Probe with Disease-Activated Near-Infrared Fluorescence, Photoacoustic, and Photothermal Signals for Imaging-Guided Therapy.
Authors: Zhen, Xu and Zhang, Jianjian and Huang, Jiaguo and Xie, Chen and Miao, Qingqing and Pu, Kanyi
Journal: Angewandte Chemie (International ed. in English) (2018): 7804-7808

Impact of plasma protein binding on cargo release by thermosensitive liposomes probed by fluorescence correlation spectroscopy.
Authors: Mittag, Judith J and Kneidl, Barbara and Preiβ, Tobias and Hossann, Martin and Winter, Gerhard and Wuttke, Stefan and Engelke, Hanna and Rädler, Joachim O
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenst (2017): 215-223

Small quinolinium-based enzymatic probes via blue-to-red ratiometric fluorescence.
Authors: Wang, Pan and Du, Jiajun and Liu, Huijing and Bi, Guoqiang and Zhang, Guoqing
Journal: The Analyst (2016): 1483-7

Isolation and Fluorescence-Activated Cell Sorting of Mouse Keratinocytes Expressing β-Galactosidase.
Authors: Kasper, Maria and Toftgård, Rune and Jaks, Viljar
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 123-36

Sensitive β-galactosidase-targeting fluorescence probe for visualizing small peritoneal metastatic tumours in vivo.
Authors: Asanuma, Daisuke and Sakabe, Masayo and Kamiya, Mako and Yamamoto, Kyoko and Hiratake, Jun and Ogawa, Mikako and Kosaka, Nobuyuki and Choyke, Peter L and Nagano, Tetsuo and Kobayashi, Hisataka and Urano, Yasuteru
Journal: Nature communications (2015): 6463

Quantitative Fluorescence Assays Using a Self-Powered Paper-Based Microfluidic Device and a Camera-Equipped Cellular Phone.
Authors: Thom, Nicole K and Lewis, Gregory G and Yeung, Kimy and Phillips, Scott T
Journal: RSC advances (2014): 1334-1340

A fluorescence-based method coupled with Disruptor filtration for rapid detection of F + RNA phages.
Authors: Yang, Y and Griffiths, M W
Journal: Letters in applied microbiology (2014): 177-83