Name: xtraFluor™ Violet 420 Maleimide
Brand: AAT Bioquest
SKU: 70802
Availability: InStock

xtraFluor™ Violet 420 Maleimide

xtraFluor™ Violet 420 Maleimide enables thiol-selective antibody labeling with exceptional brightness for violet laser flow cytometry applications.

Spectral Profile: Ex/Em ≈ 405/420 nm; optimized for the 405 nm violet laser
Superior Dim Population Resolution: Brighter than Pacific Blue™ and BD Horizon™ V450, direct spectral match to BV421™
Enhanced Stability Profile: Resists photobleaching and maintains fluorescence in standard buffers
Maleimide Chemistry: Enables selective conjugation of antibodies

(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with CD4 (RPA-T4) antibody (Cat. #10041000) labeled using xtraFluor™ Violet 420 Maleimide (Cat. #70803). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the V1-A channel, demonstrating clear detection of CD4⁺ lymphocytes.

Protocol

COA

Catalog	Size	Price	Quantity
70802	100 ug	Price
70803	1 mg	Price

Overview

xtraFluor™ Violet 420 (XV420) Maleimide is a thiol-reactive XV420 dye that can be readily used for labeling antibodies and other thiol-containing molecules such as peptides and thiol-modified oligos. XV420 is an extremely bright violet laser-excitable fluorophore. With a maximum excitation well matched the 405 nm violet laser and an emission peak at ~420 nm, XV420 can be conveniently used to replace BD Horizon Brilliant™ Violet 421 (BV421). It can be used on flow cytometers equipped with a violet laser and appropriate filters for large multicolor flow cytometry panels. It is a brighter alternative to Pacific Blue (ThermoFisher) and BD Horizon™ V450 (BD Biosciences). XV420 enables improved resolution for dim population identification. It is particularly useful in multicolor applications where it can be used to better resolve dim populations. XV420 dye offers new options for multicolor panel design through the use of a violet laser. Additionally, the dye's spillover properties, stability under light and in standard buffers, and compatibility with blood collection tubes offer ease-of-use for a range of applications.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of xtraFluor™ Violet 420 Maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	1 µL	5 µL	10 µL	50 µL	100 µL
5 mM	200 nL	1 µL	2 µL	10 µL	20 µL
10 mM	100 nL	500 nL	1 µL	5 µL	10 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	100000 g/mol	=	mL	x	mM	=	mmol

References

View all 16 references: Citation Explorer

Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy.

Authors:

Sahi, Maryam and Andersson, Sarah and Mattson, Cecilia and Dale, Matilda and Kagiolglou, Sofia and Hofström, Camilla and Persson, Helena and Klingström, Jonas and Chiodi, Francesca and Fredolini, Claudia

Journal:

Journal of visualized experiments : JoVE (2024)

Development of a Duplex Serological Multiplex Assay for the Simultaneous Detection of Epstein-Barr Virus IgA and IgG Antibodies in Nasopharyngeal Carcinoma Patients.

Authors:

Schieber, Jennifer and Pring, Miranda and Ness, Andy and Liu, Zhiwei and Hsu, Wan-Lun and Brenner, Nicole and Butt, Julia and Waterboer, Tim and Simon, Julia

Journal:

Cancers (2023)

Infection with Influenzavirus A in a murine model induces epithelial bronchial lesions and distinct waves of innate immune-cell recruitment.

Authors:

Rivière, Frédéric and Burger, Julien and Lefèvre, François and Garnier, Annabelle and Vigne, Clarisse and Tournier, Jean-Nicolas and Billon-Denis, Emmanuelle

Journal:

Frontiers in immunology (2023): 1241323

Anti-PD-1 immune-related adverse events are associated with high therapeutic antibody fixation on T cells.

Authors:

Gazzano, Marianne and Parizot, Christophe and Psimaras, Dimitri and Vozy, Aurore and Baron, Marine and Abbar, Baptiste and Fallet, Vincent and Litvinova, Elena and Canellas, Anthony and Birzu, Cristina and Pourcher, Valérie and Touat, Mehdi and Weiss, Nicolas and Demeret, Sophie and Roos-Weil, Damien and Spano, Jean-Philippe and Lebbe, Celeste and Salem, Joe-Elie and Cadranel, Jacques and Hervier, Baptiste and Gorochov, Guy and Guihot, Amélie

Journal:

Frontiers in immunology (2022): 1082084

iCoreDrop: A robust immune monitoring spectral cytometry assay with six open channels for biomarker flexibility.

Authors:

Jensen, Holly A and Kim, Jeong

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2022)

Physical properties

Molecular weight	100000
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.067
Extinction coefficient (cm ^-1 M ^-1)	3,000,000
Excitation (nm)	407
Emission (nm)	424

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on May 7, 2026