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xtraFluor™ Violet 420 Labeling Dye
xtraFluor™ Violet 420 Labeling Dye is an exceptionally bright violet laser-excitable fluorophore for multicolor flow cytometry, outperforming Pacific Blue and BV421.
  • Spectral profile: Ex/Em ≈ 405/420 nm; optimized for the 405 nm violet laser
  • Superior Dim Population Resolution: Brighter than Pacific Blue™ and BD Horizon™ V450, direct spectral match to BV421™
  • Buccutite™ Conjugation Chemistry: Room temperature, neutral pH labeling achieves >90% antibody conjugation efficiency
  • Enhanced Stability Profile: Resists photobleaching and maintains fluorescence in standard buffers
Top) The spectral profile was obtained using a 4-laser spectral flow cytometer equipped with spatially separated excitation sources at 355 nm, 405 nm, 488 nm, and 640 nm. Each laser generated a distinct emission pattern, which was computationally unmixed to produce the composite spectral signature of the fluorophore. Bottom) Flow cytometric analysis of human whole blood stained with xtraFluor™ Violet 420–conjugated anti-human CD4 antibody (clone RPA-T4, 0.25 µg/test). Fluorescence emission was detected in the V1-A channel using the Aurora spectral flow cytometer.
Top) The spectral profile was obtained using a 4-laser spectral flow cytometer equipped with spatially separated excitation sources at 355 nm, 405 nm, 488 nm, and 640 nm. Each laser generated a distinct emission pattern, which was computationally unmixed to produce the composite spectral signature of the fluorophore. Bottom) Flow cytometric analysis of human whole blood stained with xtraFluor™ Violet 420–conjugated anti-human CD4 antibody (clone RPA-T4, 0.25 µg/test). Fluorescence emission was detected in the V1-A channel using the Aurora spectral flow cytometer.
protocol
Protocol
COA
CatalogSize
Price
Quantity
70800100 ug
Price
708011 mg
Price
 
Physical properties
SolventDMSO
Spectral properties
Correction factor (280 nm)0.067
Extinction coefficient (cm -1 M -1)
3,000,000
Excitation (nm)407
Emission (nm)424
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Page updated on February 7, 2026