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Cell Surface Staining for Whole Blood Samples

  1. Collect whole blood into tubes with anti-coagulant like heparin or EDTA.
  2. Aliquot 100 μL unlysed whole blood for each antibody staining experiment or controls.
  3. Add 5 μL (0.5 μg) antibody conjuga...
2026-01-02
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Below is an example protocol for conjugation of antibodies with APC, PE, PerCP or their respective tandem dyes. The protocol provides general guidelines; actual conjugation steps may require further optimization depending on the antibodies and labels...
2026-01-02
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Conjugation Protocol

1. Reduction of Antibody

  1. Prepare a fresh solution of 1.0 M DTT (15.4 mg/100 µl) in distilled water. Antibody solutions should be at 1 mg/ml or higher for best results. The reduction can be carried out in different buffers...
2026-01-02
Technical Overview
LysoTracker dyes: Weakly basic fluorescent probes that accumulate in acidic organelles for live cell imaging of lysosomes and endosomes
2025-01-06
Technical Overview
RiboGreen dye binds RNA to enhance fluorescence for quantification in molecular biology and biopharmaceutical applications
2025-01-06

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