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Fluorescent Probe Technologies Suitable for Multicolor Spectral Flow Cytometry
Flow cytometry is a versatile tool that has applications in numerous biomedical research disciplines, including cancer biology, immunology, infectious disease monitoring, microbiology, and molecular biology. Instrumentation for performing flow cytometry has advanced considerably over the past several decades. Systems incorporating multiple lasers are commonplace, as are specialized instruments designed for particular applications, such as systems using 96-well sample loaders, systems combining microscopy with flow cytometry, and systems combining mass spectrometry with flow cytometry. Spectral flow cytometry measures the entire fluorescence emission spectra for each fluorophore deployed in a multicolor labeling experiment to produce unique spectral fingerprints. Subsequently, each spectrum is unmixed to provide a pure signal for each fluorophore. Such complete spectral analysis is beginning to supplant conventional photomultiplier tube (PMT)-based approaches as the preferred detection approach for high-dimensional multicolor flow cytometry. This technology shift provides an excellent opportunity to implement comprehensive, high-performance fluorophore families spanning the UV-visible-NIR light spectrum. Read latest issue ...


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