AAT Bioquest

AssayWise Letters 2014, Vol. 3(2)

Glycerol Assays
Glycerol is a precursor for synthesis of triglycerides and phospholipids in liver and adipose tissue. When fasting, triglycerides stored in these lipid droplets can be hydrolyzed to generate free glycerol and fatty acids.

Glycerol 3-Phosphate Assays
Glycerol 3-phosphate is an important intermediate in the glycolysis metabolic pathway. Animals, fungi, and plants use glycerol 3-Phosphate to produce ATP.

Acetylcholinesterase Assays
Acetylcholinesterase (AChE) is one of the most crucial enzymes for nerve response and function.

Nucleic Acid Quantification
Helixyte™ Green Fluorimetric dsDNA Assay Kits (Cat# 17650 & 17651) simplify DNA quantification without sacrificing sensitivity.

Multicolor Intracellular Calcium Detection Probes
Cal-520™ AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to background ratio and intracellular retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM).

Intracellular Nitric Oxide (NO) Assays
Altered NO production is implicated in various immunological, cardiovascular, neurodegenerative and inflammatory diseases. As a free radical, NO is rapidly oxidized and exists in relatively low concentration.

Intracellular Total ROS Activity Assays
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen (such as superoxide, hydroxyl radical, singlet oxygen and peroxides). ROS is highly reactive due to the presence of unpaired valence shell electrons.

iFluor™ 700 Dyes
Spectrally similar to Alexa Fluor® 700 dyes, iFluor™ 700 dyes have fluorescence emission maximum at 710 nm with fluorescence quantum yields close to 0.2.

HRP Antibody Labeling Using Buccutite™ Crosslinking Technology
Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins.

6-JOE, a xanthene dye, refers to 6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein. 6-JOE fluoresces in the yellow region of the visible spectrum and can be effectively quenched by BHQ®-1 or our TQ2 dye.