Products
Services
Resources
Selection Guides
About
iFluor® 700 Dyes
Spectrally similar to Alexa Fluor® 700 dyes, iFluor® 700 dyes have fluorescence emission maximum at 710 nm with fluorescence quantum yields close to 0.2. Compared to Alexa Fluor® 700 dyes, iFluor® 700 dyes are brighter with stronger absorption at 633 nm. Fluorescence emission of iFluor® 700 dyes is well separated from that of other commonly used red fluorophores, such as TAMRA, R-phycoerythrin and iFluor® 647 dyes, making it ideal for three or four-color labelings. iFluor® 700 dyes can be effectively excited by the 633 nm red laser in flow cytometers, giving additional near infrared emission. iFluor® 700 dyes are also excellent acceptor dyes for allophycocyanin (APC), further facilitating multicolor flow cytometry analysis. Our in-house research indicated that the iFluor® 700-labeled antibodies generally give much higher stain index than the corresponding antibodies labeled with Alexa Fluor® 700 (See Figure 2).
Fig. 1
The excitation and emission spectra of iFluor® 700 Goat Anti-Rabbit IgG Conjugate (Catalog Number 16652) in PBS buffer (pH 7.2).
Fig. 2
Left: Stain index comparison of iFluor® 700 with Alexa Fluor® 700. Right: Photostability comparison of APC-iFluor® 700 Tandem (Catalog Number 2570) with the spectrally equivalent APC-Alexa Fluor® 700 tandem in PBS buffer (pH 7.2). Both iFluor® 700 and Alexa Fluor® tandems were irradiated with 200 W lamp in PBS (pH 7.2).
This document (03.0066.141001r1) was last updated on Mon Oct 13 2025. All trademarks and registered trademarks mentioned herein are the property of their respective owners.
iFluor® 700 Dyes