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iFluor™ 700 Dyes


The excitation and emission spectra of iFluor® 700 Goat Anti-Rabbit IgG Conjugate (Cat# 16652) in PBS buffer (pH 7.2).
Spectrally similar to Alexa Fluor® 700 dyes, iFluor® 700 dyes have fluorescence emission maximum at 710 nm with fluorescence quantum yields close to 0.2. Compared to Alexa Fluor® 700 dyes, iFluor® 700 dyes are brighter with stronger absorption at 633 nm. Fluorescence emission of iFluor® 700 dyes is well separated from that of other commonly used red fluorophores, such as TAMRA, R-phycoerythrin and iFluor® 647 dyes, making it ideal for three or four-color labelings. iFluor® 700 dyes can be effectively excited by the 633 nm red laser in flow cytometers, giving additional near infrared emission. iFluor® 700 dyes are also excellent acceptor dyes for allophycocyanin (APC), further facilitating multicolor flow cytometry analysis. Our in-house research indicated that the iFluor® 700-labeled antibodies generally give much higher stain index than the corresponding antibodies labeled with Alexa Fluor® 700 (See Figure 2).


Left: Stain index comparison of iFluor® 700 with Alexa Fluor® 700. Right: Photostability comparison of APC-iFluor® 700 Tandem (Cat# 2570) with the spectrally equivalent APC-Alexa Fluor® 700 tandem in PBS buffer (pH 7.2). Both iFluor® 700 and Alexa Fluor® tandems were irradiated with 200 W lamp in PBS (pH 7.2).

 

Table 1. iFluor® 700 Dyes

Cat No.
Product Name
Alternative
Ex (nm)
Em (nm)
Unit Size
Price
20077Annexin V-iFluor® 700 conjugateAlexa Fluor® 700690716100 Tests$217
2570ReadiUse™ Preactivated APC-iFluor® 700 TandemAlexa Fluor® 7006517131 mg$382
16494iFluor® 700 goat anti-mouse IgG (H+L)Alexa Fluor® 700693713200 ug$84
16574iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed*Alexa Fluor® 700693713200 ug$106
16652iFluor® 700 goat anti-rabbit IgG (H+L)Alexa Fluor® 700693713200 ug$84
16714iFluor® 700 goat anti-rabbit IgG (H+L) *Cross Adsorbed*Alexa Fluor® 700693713200 ug$106
1087iFluor® 700 hydrazideAlexa Fluor® 7006857101 mg$217
1067iFluor® 700 maleimideAlexa Fluor® 7006857101 mg$217
16970iFluor® 700-streptavidin conjugateAlexa Fluor® 700693713200 ug$84
1036iFluor® 700 succinimidyl esterAlexa Fluor® 7006857101 mg$217