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iFluor® 700 goat anti-mouse IgG (H+L)
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 700 goat anti-mouse IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~693 nm and ~713 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 700 goat anti-mouse IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).
<p>HL-60 cells were incubated with (Red, +) or without (Green, -) Anti-human HLA-ABC (W6/32 mAb), followed by iFluor® 700 goat anti-mouse IgG conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC channel.</p>
<p>HL-60 cells were incubated with (Red, +) or without (Green, -) Anti-human HLA-ABC (W6/32 mAb), followed by iFluor® 700 goat anti-mouse IgG conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC channel.</p>
protocol
Protocol
COA
CatalogSize
Price
Quantity
16494200 ug
Price
167461 mg
Price
 
Physical properties
Molecular weight~150000
SolventWater
Spectral properties
Correction factor (260 nm)0.09
Correction factor (280 nm)0.04
Correction factor (565 nm)0.0766
Correction factor (650 nm)0.4004
Extinction coefficient (cm -1 M -1)
220000
1
Excitation (nm)690
Emission (nm)713
Quantum yield
0.23
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 8, 2025