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AssayWise Letters 2015, Vol. 4(1)
In this issue
Fluorescent Calcium Indicators
Fluo-8® Calcium Indicators
Cal-520™ Calcium Indicators
Cal-590™ Calcium Indicators
Cal-630™ Calcium Indicators
Rhod-4™ Calcium Indicators
Ratiometric Calcium Indicators
FLIPR® Calcium Assays
AssayWise Letters 2015, Vol. 4(1)
Featured Article
Fluorescent Calcium Indicators
Calcium acts as a universal second messenger in a variety of cells. Numerous functions of all types of cells are regulated by Ca2+, thus calcium measurement is critical for various biological investiga-tions.…
Calcium acts as a universal second messenger in a variety of cells. Numerous functions of all types of cells are regulated by Ca2+, thus calcium measurement is critical for various biological investiga-tions.…
Fluo-8® Calcium Indicators
Fluo-3 and Fluo-4 were the most commonly used visible light-excitable calcium indicators. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydro-lysis, and require harsh cell loading conditions to maximize their cellular calcium responses.…
Fluo-3 and Fluo-4 were the most commonly used visible light-excitable calcium indicators. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydro-lysis, and require harsh cell loading conditions to maximize their cellular calcium responses.…
Cal-520™ Calcium Indicators
Cal-520™ provides the most robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization.…
Cal-520™ provides the most robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization.…
Cal-590™ Calcium Indicators
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence…
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence…
Cal-630™ Calcium Indicators
X-Rhod-1 is commonly used as a red fluorescent calcium indicator. However, X-Rhod-1 is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses.…
X-Rhod-1 is commonly used as a red fluorescent calcium indicator. However, X-Rhod-1 is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses.…
Rhod-4™ Calcium Indicators
Rhod-2 is the most commonly used red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses.…
Rhod-2 is the most commonly used red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses.…
Ratiometric Calcium Indicators
Among the ratiometric calcium indicators, Fura-2 and Indo-1 are most commonly used. BTC is another excitation-ratioable calcium indicator.…
Among the ratiometric calcium indicators, Fura-2 and Indo-1 are most commonly used. BTC is another excitation-ratioable calcium indicator.…