Cal-520™ Calcium Indicators

Left: ATP-stimulated calcium responses of endogenous P2Y receptor in CHO-K1 cells incubated with Cal-520™ AM (red curve, Cat# 21131), or Fluo-4 AM (blue curve) respectively with probenecid under the same conditions. CHO-K1 cells were seeded overnight at 50,000 cells/100 µL/well in a Costar 96-well black wall/clear bottom plate. 100 µL of 5 µM Fluo-4 AM or Cal-520™ AM in HHBS with 2.5 mm probenecid was added into the cells, and the cells were incubated at 37 °C for 2 hours. ATP (50 µL/well) was added using FlexSation^® to achieve the final indicated concentrations. Right: ATP-stimulated calcium responses of endogenous P2Y receptors in CHO-K1 cells incubated with Cal-520™ AM (red curve, Cat# 21131), or Fluo-4 AM (blue curve) respectively, without probenecid under the same conditions. CHO-K1 cells were seeded overnight at 50,000 cells/100 µL/well in a Costar 96-well black wall/clear bottom plate. 100 µL of 5 µM Fluo-4 AM or Cal-520™ AM in HHBS was added into the cells, and the cells were incubated at 37 °C for 2 hours. ATP (50 µL/well) was added using FlexSation^® to achieve the final indicated concentrations.