AAT Bioquest

AssayWise Letters 2021, Vol. 10(1)

Enzymatic and Chemical Labeling Strategies for Oligonucleotides
Labeling strategies for oligonucleotides (oligos) differ somewhat from those for other commonly encountered biopolymers, such as proteins and carbohydrates, since oligos are exceptionally inert to common bioconjugation reagents under standard reaction conditions. While reagents are available

Sensitive Identification of Newly Synthesized DNA In Situ using click chemistry-based Bucculite™ XdU Cell Proliferation Assays
The measurement of cell proliferation in situ is fundamental to the assessment of cell health, genotoxicity, and drug efficacy evaluation. Precisely labeling and quantifying cells in the synthesis phase (S phase) of cell cycle progression is not only adequate for characterizing basic biology but

A Practical Guide for the Detection and Analysis of PCR Products
PCR, or polymerase chain reaction, is a core technology used routinely to amplify a specific DNA sequence rapidly. Offering exceptional speed, specificity, and sensitivity as a tool, PCR, and its many adaptations, such as real-time PCR and reverse transcription-PCR, have revolutionized how DNA