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AAT Bioquest

AssayWise Letters 2016, Vol. 5(1)

Horseradish Peroxidase (HRP)
Horseradish Peroxidase is the ideal enzyme for use in techniques such as ELISA and immunohistochemistry due to it's monomeric nature and it's ease in producing colored products.

ReadiLink™ Antibody Labeling Technology
ReadiLink™ iFluor™ Antibody Labeling Kits provide the best method for labeling microscale volumes of antibodies (proteins > 10kDa). ReadiLink™ kits allow you to quickly label your antibody of interest in two easy steps without requiring column purification.

ReadiView™ Biotinylation Technology
ReadiView™ biotin contains a specially designed color tag that enables biotinylation degree to be easily calculated using the correct absorption rate (280nm/385nm). This color tag has minimal effect on the biotin binding affinity and reduced quenching effect on most fluorophores that are used

TR-FRET Assays
TR-FRET Assays are a combination of time-resolved fluorometry (TRF) and Förster resonance energy transfer (FRET) technology that results in a much more flexible, reliable, and sensitive assay with higher throughput and less false positive/negative results.

RNA Detection
StrandBrite™ RNA Green has addressed the challenge of analyzing RNA in live cells with little DNA interference. StrandBrite™ RNA Green is a sensitive RNA-selective dye used to stain nucleolar RNA in live and fixed cells with less DNA interference than commonly used SYTO® RNASelect™ dye. StrandBrite™


Calcein-Based Cell Viability Assays
Calcein, a fluorescein derivative, is commonly used in cell viability assays due to it's low cytotoxicity, enhanced sensitivity, and greater range of sensitivity in comparison to colorimetric assays such as MTT. Another valuable attribute of Calcein assays is that they are able to be completed in

Fluorescence Live Cell Imaging
Cell Navigator™ Live Cell Endoplasmic Reticulum (ER) Staining Kit 22635 uses ER Tracer™ Green. This stain is a cell-permeant fluorescent dye that is highly selective for ER. This stain consists of a green fluorescent dye and ER binder that selectively bind to ER in most cell types. You also have the option