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Fluorescence Live Cell Imaging

The endoplasmic reticulum (ER) is a type of organelle in the cells of eukaryotic organisms that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae. The membranes of the ER are continuous with the outer nuclear membrane. ER occurs in most types of eukaryotic cells, but is absent from red blood cells and spermatozoa.

Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit 22635 uses our ER Tracer™ Green as an ER marker. ER Tracer™ Green stain is a cell-permeant fluorescent dye that is highly selective for ER. This stain consists of a green fluorescent dye and ER binder that selectively bind to ER in most of cell types. For some cells, ER Tracer™ Green may not selectively bind to ER. ER Tracer™ Green has spectral properties essentially identical to FITC, making this kit convenient with the FITC filter set.


Fluorescence images of endoplasmic reticulum (ER) staining in HeLa cells cultured in a 96-well black wall/clear bottom plate. Left: Live cells were stained with Cell Navigator® Live Cell Endoplasmic Reticulum Staining Kit (Cat# 22635, Green), mitochondria dye MitoLite™ Red FX600 (Cat# 22677, Red) and nuclei stain Hoechst 33342 (Cat# 17530, Blue). Right: Live cells stained with Kit 22635 (Green) were fixed with 4% formaldehyde, and labeled with F-actin dye Phalloidin-iFluor® 594 (Cat# 23122, Red) and nuclei stain DAPI (Cat# 17507, Blue).


Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit 22636 uses our ER Tracer™ Red as an ER marker. ER Tracer™ Red stain is a cell-permeant fluorescent dye that is highly selective for ER. This stain consists of a red fluorescent dye and ER binder that selectively bind to ER in most of cell types. For some cells, ER Tracer™ Red may not selectively bind to ER. ER Tracer™ Red has spectral properties essentially identical to Texas Red®, making this kit convenient with the Texas Red® filter set.


Fluorescence images of endoplasmic reticulum (ER) staining in HeLa cells cultured in a 96-well black wall/clear bottom plate. Left: Live cells were stained with Cell Navigator® Live Cell Endoplasmic Reticulum Staining Kit (Cat# 22636, Red), mitochondria dye MitoLite™ Green (Cat# 22675, Green) and nuclei stain Hoechst 33342 (Cat# 17530, Blue). Right: Live cells stained with Kit 22636 (Red) were fixed with 4% formaldehyde, then labeled with F-actin dye Phalloidin-iFluor® 488 (Cat# 22661, Green) and nuclei stain DAPI (Cat# 17507, Blue).

 

Table 1. Cell Navigator® ER Staining Kits for imaging the endoplasmic reticulum of live cells.

Kit
Probe
Ex/Em (nm)
Filter Set
Unit Size
Cat No.
Cell Navigator® Live Cell ER Staining Kit *Blue Fluorescence*ER Tracer™ Blue344/456DAPI100 Tests22634
Cell Navigator® Live Cell ER Staining Kit *Green Fluorescence*ER Tracer™ Green503/511FITC100 Tests22635
Cell Navigator® Live Cell ER Staining Kit *Red Fluorescence*ER Tracer™ Red589/620Texas Red100 Tests22636



The fluorescence image of HeLa cells stained with Cell Navigator® Cell Plasma Membrane Staining Kit (Cat# 22680) in a 96-well black wall/clear bottom plate. The cells were imaged using a fluorescence microscope with a TRITC filter.
The cell membrane (plasma membrane) is a thin semi-permeable membrane that separates the interior of all cells from the environment. Cell membranes are involved in a variety of cellular processes, such as cell adhesion, ion conductivity and cell signaling, and serve as the attachment surface for several extracellular structures, including the cell wall, glycocalyx, and intracelluar cytoskeleton.

Cell Navigator® Cell Plasma Membrane Staining Kits (Cat# 22680 & 22681) provide a fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. The kits may be used as a segmentation tool for HCS (high-content screening). They can also be used to stain cellular plasma membranes for standard fluorescence microscopy. The cell membrane probes used in the kits survive fixation, but not permeabilization, so they are not suitable for experiments that also involve probing internal targets via antibodies.
 

Table 2. Cell Navigator® Plasma Membrane Staining kits for live cells.

Product Name
Probe
Ex/Em (nm)
Filter Set
Unit Size
Cat No.
Cell Navigator® Cell Plasma Membrane Staining Kit *Green Fluorescence*Cellpaint™ Green497/505FITC500 tests22682
Cell Navigator® Cell Plasma Membrane Staining Kit *Orange Fluorescence*Cellpaint™ Orange555/573TRITC500 tests22680
Cell Navigator® Cell Plasma Membrane Staining Kit *Red Fluorescence*Cellpaint™ Red648/671Cy5500 tests22681