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MitoLite™ Green EX488
MitoLite™ Green is a cationic dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for long time since the indicator carries a cell-retaining group. This key feature significantly increases its staining efficiency. The labeling protocol is robust, requiring minimal hands-on time. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Fluorescence image of HeLa cells stained with MitoLite™ Green EX488 in a Costar black-wall/clear bottom 96-well plate.
Fluorescence image of HeLa cells stained with MitoLite™ Green EX488 in a Costar black-wall/clear bottom 96-well plate.
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
22675500 Tests
Price
 
Physical properties
Molecular weightN/A
SolventDMSO
Spectral properties
Excitation (nm)508
Emission (nm)528
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 8, 2025