AAT Bioquest

MitoLite™ Dyes and Kits

Live Cell Mitochondrial Imaging

MitoLite™ dyes are a series of cell-permeable cationic dyes used to label mitochondria in living cells. At micromolar concentrations (µM), MitoLite™ dyes passively diffuse across live cell membranes and selectively accumulate in active mitochondria via the mitochondrial membrane potential (ΔΨm) gradient. Since staining is lost during cell death due to the collapse (depolarization) of the mitochondrial membrane potential, MitoLite™ dyes can also be used to monitor cell viability. MitoLite™ dyes are available in spectral characteristics ranging from blue to near-infrared to accommodate multiplexing with other fluorescent probes or after fixation for co-localization studies. Retention after fixation varies amongst MitoLite™ dyes, with dyes such as MitoLite™ Blue FX490 being well-retained and MitoLite™ Green FM being lost after fixation. MitoLite™ dyes are key components of Cell Navigator™ Mitochondrion Staining Kits.

Cell Navigator™ Mitochondrion Staining Kits combine the enhanced staining efficiency of MitoLite™ reagents with an optimized staining protocol to rapidly label mitochondria in living cells. Each kit supplies sufficient materials for performing 500 assays. Cell Navigator™ Mitochondrion Staining Kits can be readily adapted for various fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. They are effective in a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity.

Features of MitoLite™ probes:

  • Selectively accumulates in active mitochondria via the ΔΨm gradient
  • Exceptionally bright fluorescence with high signal-to-noise ratios
  • Photostable signal generation for extended imaging windows
  • Can be imaged without washing
  • Multiple wavelength options for multiplex analysis with other fluorescent probes
  • Suitable for both proliferating and non-proliferating cells, as well as, cell in suspension and adherent cells


MitoLite™ Images


Table 1. Available MitoLite™ dyes for labeling mitochondria in live cells.

Product name
Sample Type
Ex (nm)
Em (nm)
Filter Set
Unit Size
Cat No.
Assay Kit No.
MitoLite™ Blue FX490Live CellsYes344469DAPI500 Tests2267422665
MitoLite™ Green FMLive CellsNo508528FITC10x50 µg22695-
MitoLite™ Green EX488Live CellsNo508528FITC500 Tests2267522666
MitoLite™ Orange EX405Live CellsNo425522FITC500 Tests2267922673
MitoLite™ Orange FX570Live CellsYes553576TRITC500 Tests2267622667
MitoLite™ Red FX600Live CellsYes580598TRITC500 Tests22677-
MitoLite™ Red CMXRosLive CellsYes579599TRITC10x50 µg2269822668
MitoLite™ Deep Red FX660Live CellsYes640659Cy5500 Tests2267822669
MitoLite™ NIR FX690Live CellsYes658691Cy5500 Tests2269022670


Fluorescence Spectrum Viewer

Need assistance selecting the best fluorophore for your experiment, use our Fluorescence Spectrum Viewer:
  • View and compare fluorophores and fluorescent proteins for biological applications
  • Check spectral compatibility
  • Add multiple excitation and emission filters
  • Save spectra configuration as a PNG or hyperlink