MitoLite™ Blue FX490

MitoLite™ Blue is a cationic dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for long time since the indicator carries a cell-retaining group. This key feature significantly increases its staining efficiency. The labeling protocol is robust, requiring minimal hands-on time. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.

Image of HeLa cells stained with the MitoLilte™ Blue FX490 in a 96-well clear-bottom plate.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22674	500 Tests	Price

Citations

View all 8 citations: Citation Explorer

Mitofusin-mediated contacts between mitochondria and peroxisomes regulate mitochondrial fusion

Authors:

Alsayyah, Cynthia and Singh, Manish K and Morcillo-Parra, Maria Angeles and Cavellini, Laetitia and Shai, Nadav and Schmitt, Christine and Schuldiner, Maya and Zalckvar, Einat and Mallet, Adeline and Belgareh-Touz{\'e}, Na{\"\i}ma and others,

Journal:

PLoS biology (2024): e3002602

A selective fluorescent turn-on probe for imaging and sensing of hydrogen peroxide in living cells

Authors:

Ciou, Jyun-Jia and Wu, Tzu-Chien and Chen, Zhi-Jia and Cheng, Bill and Lai, Chian-Hui

Journal:

Analytical and Bioanalytical Chemistry (2023): 1--11

Cetyltrimethylammonium chloride-loaded mesoporous silica nanoparticles as a mitochondrion-targeting agent for tumor therapy

Authors:

Tang, Menghuan and Zhang, Peng and Liu, Jiahui and Long, Yijuan and Cheng, Yuan and Zheng, Huzhi

Journal:

RSC Advances (2020): 17050--17057

A ratiometric fluorescent probe for detecting hypochlorite in the endoplasmic reticulum

Authors:

Hou, Ji-Ting and Kim, Hyeong Seok and Duan, Chong and Ji, Myung Sun and Wang, Shan and Zeng, Lintao and Ren, Wen Xiu and Kim, Jong Seung

Journal:

Chemical communications (2019): 2533--2536

Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer

Authors:

Heger, Zbynek and Polanska, Hana and Krizkova, Sona and Balvan, Jan and Raudenska, Martina and Dostalova, Simona and Moulick, Amitava and Masarik, Michal and Adam, Vojtech

Journal:

Colloids and Surfaces B: Biointerfaces (2017): 131--140

References

View all 70 references: Citation Explorer

Quantification of carbonylated proteins in rat skeletal muscle mitochondria using capillary sieving electrophoresis with laser-induced fluorescence detection

Authors:

Feng J, Arriaga EA.

Journal:

Electrophoresis (2008): 475

Calcium, mitochondria and apoptosis studied by fluorescence measurements

Authors:

Roy SS, Hajnoczky G.

Journal:

Methods (2008): 213

Fluorescence imaging of mitochondria in yeast

Authors:

Swayne TC, Gay AC, Pon LA.

Journal:

Methods Mol Biol (2007): 433

A fluorescence assay for peptide translocation into mitochondria

Authors:

Martinez-Caballero S, Peixoto PM, Kinnally KW, Campo ML.

Journal:

Anal Biochem (2007): 76

Fast electrophoretic analysis of individual mitochondria using microchip capillary electrophoresis with laser induced fluorescence detection

Authors:

Duffy CF, MacCraith B, Diamond D, O'Kennedy R, Arriaga EA.

Journal:

Lab Chip (2006): 1007

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Excitation (nm)	344
Emission (nm)	469

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 8, 2025