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MitoLite™ Blue FX490

Image of HeLa cells stained with the MitoLilte™ Blue FX490 in a 96-well clear-bottom plate.
Image of HeLa cells stained with the MitoLilte™ Blue FX490 in a 96-well clear-bottom plate.
Image of HeLa cells stained with the MitoLilte™ Blue FX490 in a 96-well clear-bottom plate.
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Physical properties
Molecular weightN/A
Spectral properties
Excitation (nm)344
Emission (nm)469
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
MitoLite™ Blue is a cationic dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for long time since the indicator carries a cell-retaining group. This key feature significantly increases its staining efficiency. The labeling protocol is robust, requiring minimal hands-on time. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.


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Spectral properties

Excitation (nm)344
Emission (nm)469



View all 8 citations: Citation Explorer
Mitofusin-mediated contacts between mitochondria and peroxisomes regulate mitochondrial fusion
Authors: Alsayyah, Cynthia and Singh, Manish K and Morcillo-Parra, Maria Angeles and Cavellini, Laetitia and Shai, Nadav and Schmitt, Christine and Schuldiner, Maya and Zalckvar, Einat and Mallet, Adeline and Belgareh-Touz{\'e}, Na{\"\i}ma and others,
Journal: PLoS biology (2024): e3002602
A selective fluorescent turn-on probe for imaging and sensing of hydrogen peroxide in living cells
Authors: Ciou, Jyun-Jia and Wu, Tzu-Chien and Chen, Zhi-Jia and Cheng, Bill and Lai, Chian-Hui
Journal: Analytical and Bioanalytical Chemistry (2023): 1--11
Cetyltrimethylammonium chloride-loaded mesoporous silica nanoparticles as a mitochondrion-targeting agent for tumor therapy
Authors: Tang, Menghuan and Zhang, Peng and Liu, Jiahui and Long, Yijuan and Cheng, Yuan and Zheng, Huzhi
Journal: RSC Advances (2020): 17050--17057
A ratiometric fluorescent probe for detecting hypochlorite in the endoplasmic reticulum
Authors: Hou, Ji-Ting and Kim, Hyeong Seok and Duan, Chong and Ji, Myung Sun and Wang, Shan and Zeng, Lintao and Ren, Wen Xiu and Kim, Jong Seung
Journal: Chemical communications (2019): 2533--2536
Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer
Authors: Heger, Zbynek and Polanska, Hana and Krizkova, Sona and Balvan, Jan and Raudenska, Martina and Dostalova, Simona and Moulick, Amitava and Masarik, Michal and Adam, Vojtech
Journal: Colloids and Surfaces B: Biointerfaces (2017): 131--140
Inhibition of heme oxygenase-1 enhances the chemosensitivity of laryngeal squamous cell cancer Hep-2 cells to cisplatin
Authors: Lv, Xin and Song, Dong-mei and Niu, Ying-hao and Wang, Bao-shan
Journal: Apoptosis (2016): 489--501
Effective two-photon excited photodynamic therapy of xenograft tumors sensitized by water-soluble bis (arylidene) cycloalkanone photosensitizers
Authors: Zou, Qianli and Zhao, Hongyou and Zhao, Yuxia and Fang, Yanyan and Chen, Defu and Ren, Jie and Wang, Xiaopu and Wang, Ying and Gu, Ying and Wu, Feipeng
Journal: Journal of medicinal chemistry (2015): 7949--7958
Melatonin promotes adipogenesis and mitochondrial biogenesis in 3T3-L1 preadipocytes
Authors: Kato, Hisashi and Tanaka, Goki and Masuda, Shinya and Ogasawara, Junetsu and Sakurai, Takuya and Kizaki, Takako and Ohno, Hideki and Izawa, Tetsuya
Journal: Journal of Pineal Research (2015): 267--275


View all 70 references: Citation Explorer
Quantification of carbonylated proteins in rat skeletal muscle mitochondria using capillary sieving electrophoresis with laser-induced fluorescence detection
Authors: Feng J, Arriaga EA.
Journal: Electrophoresis (2008): 475
Calcium, mitochondria and apoptosis studied by fluorescence measurements
Authors: Roy SS, Hajnoczky G.
Journal: Methods (2008): 213
Fluorescence imaging of mitochondria in yeast
Authors: Swayne TC, Gay AC, Pon LA.
Journal: Methods Mol Biol (2007): 433
A fluorescence assay for peptide translocation into mitochondria
Authors: Martinez-Caballero S, Peixoto PM, Kinnally KW, Campo ML.
Journal: Anal Biochem (2007): 76
Fast electrophoretic analysis of individual mitochondria using microchip capillary electrophoresis with laser induced fluorescence detection
Authors: Duffy CF, MacCraith B, Diamond D, O'Kennedy R, Arriaga EA.
Journal: Lab Chip (2006): 1007
Discrimination of depolarized from polarized mitochondria by confocal fluorescence resonance energy transfer
Authors: Elmore SP, Nishimura Y, Qian T, Herman B, Lemasters JJ.
Journal: Arch Biochem Biophys (2004): 145
A fluorescence-based technique for screening compounds that protect against damage to brain mitochondria
Authors: Kristian T, Fiskum G.
Journal: Brain Res Brain Res Protoc (2004): 176
Determination of the cardiolipin content of individual mitochondria by capillary electrophoresis with laser-induced fluorescence detection
Authors: Fuller KM, Duffy CF, Arriaga EA.
Journal: Electrophoresis (2002): 1571
Fluorescence imaging of metabolic responses in single mitochondria
Authors: Nakayama S, Sakuyama T, Mitaku S, Ohta Y.
Journal: Biochem Biophys Res Commun (2002): 23
Visualisation of mitochondria in living neurons with single- and two-photon fluorescence laser microscopy
Authors: Dedov VN, Cox GC, Roufogalis BD.
Journal: Micron (2001): 653