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ReadiLink™ Antibody Labeling Technology
ReadiLink™ iFluor® Antibody Labeling Kits provide a convenient way to label antibodies using a stable reactive form of the iFluor® dyes. The iFluor® dyes show good reactivity and selectivity with the aliphatic amines of antibodies and forms a carboxamide bond, which is identical to, and is as stable as the natural peptide bond. iFluor®-antibody conjugates may be used for immunofluorescent staining, fluorescent in situ hybridization, flow cytometry and other biological applications. Each kit comes with all the essential components for performing the conjugation reaction and for purifying the iFluor®-antibody conjugates. ReadiLink™ Kits quickly label your antibody of interest in two easy steps with the unique chemistry of ReadiLink™ Dyes. The conjugation kits provide the best method for readily labeling microscale volumes without requiring column purification.
Key Features of ReadiLink™ Kits:
- Save time with no purification
- Conjugate your antibodies in 2 easy
- Available in 10 different fluorophores with excitation wavelengths ranging from UV to infrared.
Fig. 1
ReadiLink™ Kit Labeling Principle
- Start the labeling reaction by mixing a labeling dye with a protein (to be labeled) in the reaction buffer (pH 7.5-8.5).
- Incubate the reaction solution and get a mixture of the desired protein conjugate and unreactive free dye.
- Quench the reaction by mixing a non-fluorescent Tide Quencher™ (TQ) dye with the reaction solution. The TQ dye stops the reaction AND converts the unreactive free labeling dye to the non-fluorescent TQ-labeled dye complex, which eliminates the background fluorescence interference of the free labeling dye.
Document: 03.0089.160501r1
Last updated Wed Sep 10 2025
ReadiLink™ Antibody Labeling Technology