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ReadiLink™ Antibody Labeling Technology

ReadiLink™ iFluor® Antibody Labeling Kits provide a convenient way to label antibodies using a stable reactive form of the iFluor® dyes. The iFluor® dyes show good reactivity and selectivity with the aliphatic amines of antibodies and forms a carboxamide bond, which is identical to, and is as stable as the natural peptide bond. iFluor®-antibody conjugates may be used for immunofluorescent staining, fluorescent in situ hybridization, flow cytometry and other biological applications. Each kit comes with all the essential components for performing the conjugation reaction and for purifying the iFluor®-antibody conjugates. ReadiLink™ Kits quickly label your antibody of interest in two easy steps with the unique chemistry of ReadiLink™ Dyes. The conjugation kits provide the best method for readily labeling microscale volumes without requiring column purification.

Key Features of ReadiLink™ Kits:

  • Save time with no purification
  • Conjugate your antibodies in 2 easy
  • Available in 10 different fluorophores with excitation wavelengths ranging from UV to infrared.


ReadiLink™ Kit Labeling Principle
  1. Start the labeling reaction by mixing a labeling dye with a protein (to be labeled) in the reaction buffer (pH 7.5-8.5).
  2. Incubate the reaction solution and get a mixture of the desired protein conjugate and unreactive free dye.
  3. Quench the reaction by mixing a non-fluorescent Tide Quencher™ (TQ) dye with the reaction solution. The TQ dye stops the reaction AND converts the unreactive free labeling dye to the non-fluorescent TQ-labeled dye complex, which eliminates the background fluorescence interference of the free labeling dye.

 

Table 1. ReadiLink™ iFluor® Antibody Labeling Assays

Cat No.
Product Name
Alternative to
Ex (nm)
Em (nm)
Unit Size
Price
1299ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*Lightning-Link® Dye Labeling Kits4925162 Labelings$162
1220ReadiLink™ Rapid iFluor® 350 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*Lightning-Link® Dye Labeling Kits3454422 Labelings$162
1255ReadiLink™ Rapid iFluor® 488 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*Lightning-Link® Dye Labeling Kits4915142 Labelings$162
1227ReadiLink™ Rapid iFluor® 555 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*Lightning-Link® Dye Labeling Kits5595692 Labelings$162
1230ReadiLink™ Rapid iFluor® 594 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*Lightning-Link® Dye Labeling Kits5926142 Labelings$162
1260ReadiLink™ Rapid iFluor® 633 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*Lightning-Link® Dye Labeling Kits6386552 Labelings$162
1235ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*Lightning-Link® Dye Labeling Kits6546742 Labelings$162
1240ReadiLink™ Rapid iFluor® 680 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*Lightning-Link® Dye Labeling Kits6827012 Labelings$162
1245ReadiLink™ Rapid iFluor® 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*Lightning-Link® Dye Labeling Kits6937132 Labelings$162
1250ReadiLink™ Rapid iFluor® 750 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*Lightning-Link® Dye Labeling Kits7537792 Labelings$162